OXIDASE RELATED 48K PHOSPHOPROTEIN OF HUMAN NEUTROPHILS

呼吸爆发氧化酶（RBO）是负责 吞噬细胞和B淋巴细胞产生超氧化物。它休眠在 休眠的细胞，但当细胞被激活时，它们就会复活。中 RBO的组成部分是一种47 K（以前称为48 K）蛋白质，称为p47 phox 当含有RBO的细胞被激活时就会磷酸化。的 本工作的目的是确定磷酸化位点， p47 phox，并阐明其与RBO活性的关系; 鉴定与p47 phox相互作用的蛋白质，并了解这些蛋白质 在分子水平上的相互作用;并产生大量的 p47 phox用于X射线晶体学结构测定。

The respiratory burst oxidase (RBO) is the enzyme responsible for superoxide production by phagocytes and B lymphocytes. It is dormant in resting cells but comes to life when the cells are activated. Among the components of the RBO is a 47K (formerly 48K) protein known as p47phox that becomes phosphorylated when RBO-containing cells are activated. The aims of the present work are to identify the sites of phosphorylation in p47phox and elucidate their relationship to the activity of the RBO; to identify proteins with which p47phox interacts and understand these interactions at a molecular level; and to produce large quantities of p47phox for structure determination by X- ray crystallography.