Core--Flow cytometry and fluorescence imaging facility

核心--流式细胞仪和荧光成像设备

• 批准号: 6231699
• 负责人: Alice L. Givan
• 金额: --
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6231699
• 关键词: biomedical equipment resource; biomedical facility; flow cytometry; fluorescence microscopy; neoplasm /cancer; oncology

The Flow Cytometry'/Fluorescence Imaging Shared Resource provides Cancer Center investigators with advanced instrumentation for the study of cells. To this end, it aims to provide state-of-the-art flow cytometers and image analysis systems for acquisition of data; to provide computer hardware and software for the analysis of cellular data derived from these instruments; to train scientists in use of the instruments and software; to develop and implement new techniques for cell analysis; and to encourage communication among scientists on methods of common interest. For flow cytometry, the Shared Resource has two Becton Dickinson instruments: a FACStar Plus VIS/UV seven-parameter cytometer with TurboSort upgrade for sorting and advanced applications and a FACScan five-parameter cytometer for routine analysis. The Resource laboratory also has three image analysis systems: a Bio-Rad MRC-1024 krypton/argon laser confocal system; a Meridian Ultima UV/VIS argon laser confocal system; and a Zeiss Axiophot photomicroscope with a cooled CCD detector. There are, within the Shared Resource, a Silicon Graphics workstation, three networked Macintosh workstations, and a Codonics dye~sublimation printer for publication-quality color printing. During FY 96-97, the Flow Cytometry/'Fluorescence Imaging Shared Resource was used by approximately 100 different individuals from the labs of 34 investigators. For that period, 2,741 out of a total of 2,858 billed hours of use were by Cancer Center members (95.9% of total billed use). At the present time, Cancer Center investigators using the Shared Resource hold active grants totalling $10,399,757 in annual direct costs. Of this sum, 97% is from peer-reviewed grants (84.2% from NIH).

流式细胞术/荧光成像共享资源为癌症中心的研究人员提供了先进的细胞研究仪器。为此目的，它的目标是提供最先进的流式细胞仪和图像分析系统，用于获取数据;提供计算机硬件和软件，用于分析从这些仪器获得的细胞数据;培训科学家使用仪器和软件;开发和实施细胞分析新技术;鼓励科学家就共同感兴趣的方法进行交流。对于流式细胞术，共享资源有两个Becton Dickinson仪器：一个FACStar Plus维斯/UV七参数细胞仪，带有TurboSort升级，用于分选和高级应用，一个FACScan五参数细胞仪用于常规分析。资源实验室还拥有三个图像分析系统：Bio-Rad MRC-1024氪/氩激光共聚焦系统; Meridian Ultima UV/维斯氩激光共聚焦系统;以及带有冷却CCD检测器的Zeiss Axiophot显微镜。在共享资源中，有一个硅图形工作站，三个联网的麦金塔工作站，和一个用于出版质量彩色打印的Codonics染料升华打印机。在1996 - 1997财政年度期间，来自34名研究者实验室的大约100名不同人员使用了流式细胞术/荧光成像共享资源。在此期间，在总共2，858个计费使用小时中，有2，741个是由癌症中心成员使用的（占总计费使用的95.9%）。目前，使用共享资源的癌症中心研究人员每年直接赠款总额为10，399，757美元。其中，97%来自同行评审赠款（84.2%来自NIH）。