Core--Flow cytometry and fluorescence imaging facility

流式细胞术/荧光成像共享资源为癌症中心的研究人员提供了先进的细胞研究仪器。为此，它旨在提供最先进的流式细胞仪和图像分析系统，用于获取数据；提供计算机硬件和软件，用于分析来自这些仪器的细胞数据；培训科学家使用仪器和软件；开发和实施新的细胞分析技术；并鼓励科学家之间就共同感兴趣的方法进行交流。对于流式细胞术，共享资源有两台Becton Dickinson仪器：用于分选和高级应用的FACStar Plus VIS/UV七参数细胞仪和用于常规分析的FACScan五参数细胞仪。资源实验室还拥有三个图像分析系统：Bio-Rad MRC-1024氪/氩激光共聚焦系统；子午Ultima紫外/可见氩激光共聚焦系统；以及带有冷却CCD探测器的蔡司轴向显微显微镜。在共享资源中，有一个硅图形工作站，三个联网的Macintosh工作站和一个用于出版质量彩色打印的Codonics染料升华打印机。在96-97学年，来自34名研究者实验室的大约100名不同的个体使用了流式细胞术/荧光成像共享资源。在此期间，癌症中心成员总共使用了2,858小时，其中2,741小时（占总使用时间的95.9%）。目前，使用共享资源的癌症中心研究人员在年度直接费用中持有总计10,399,757美元的积极赠款。其中97%来自同行评审拨款（84.2%来自NIH）。

The Flow Cytometry'/Fluorescence Imaging Shared Resource provides Cancer Center investigators with advanced instrumentation for the study of cells. To this end, it aims to provide state-of-the-art flow cytometers and image analysis systems for acquisition of data; to provide computer hardware and software for the analysis of cellular data derived from these instruments; to train scientists in use of the instruments and software; to develop and implement new techniques for cell analysis; and to encourage communication among scientists on methods of common interest. For flow cytometry, the Shared Resource has two Becton Dickinson instruments: a FACStar Plus VIS/UV seven-parameter cytometer with TurboSort upgrade for sorting and advanced applications and a FACScan five-parameter cytometer for routine analysis. The Resource laboratory also has three image analysis systems: a Bio-Rad MRC-1024 krypton/argon laser confocal system; a Meridian Ultima UV/VIS argon laser confocal system; and a Zeiss Axiophot photomicroscope with a cooled CCD detector. There are, within the Shared Resource, a Silicon Graphics workstation, three networked Macintosh workstations, and a Codonics dye~sublimation printer for publication-quality color printing. During FY 96-97, the Flow Cytometry/'Fluorescence Imaging Shared Resource was used by approximately 100 different individuals from the labs of 34 investigators. For that period, 2,741 out of a total of 2,858 billed hours of use were by Cancer Center members (95.9% of total billed use). At the present time, Cancer Center investigators using the Shared Resource hold active grants totalling $10,399,757 in annual direct costs. Of this sum, 97% is from peer-reviewed grants (84.2% from NIH).