Purchase of Leica TCS SP2 Confocal Microscope System

购买Leica TCS SP2共焦显微镜系统

• 批准号: 6439974
• 负责人: Alice L. Givan
• 金额: $ 36.8万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439974
• 关键词: biomedical equipment purchase; cell biology; confocal scanning microscopy

This Shared Instrumentation Grant application is for the purchase of a Leica TCS SP2 laser scanning confocal microscope system. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a Shared Core Resource of the Norris Cotton Cancer Center at Dartmouth Medical School. The Englert Laboratory was establish3d in 1981 for flow cytometry and has been providing Dartmouth with imaging as well as flow cytometric instrumentation since 1993. The new confocal instrument will replace an outdated confocal system, giving users access to the stable, flexible, and up-to-date imaging capabilities that they require. The requirement for a new confocal system is based on several urgent needs. The existing confocal system does not have the laser stability or excitation and emission flexibility required for multicolor quantitative work by a varied and large group of users. In addition, there is an expressed need for live cell studies that cannot be met by the existing upright microscope stand lacking temperature control. Finally, a new system will have the ability to acquire images with 12 bit intensity resolution, will provide regions of interest for FRAP and inverse FRAP analysis, and will provide 4 PMTs for four color fluorescence acquisition-all of which are lacking on our current system and are required now by many users. A Leica TCS SP2 system will satisfy current users of the Core Facility and will provide flexibility for adaptation to the needs of users in the future. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and an imaging supervisor. It is supported financially from the NCI core grant to the Norris Cotton Cancer Center, by hourly charges to users, and by institutional support from Dartmouth Medical School. At the present time, there are 33 NIH grant holders dependent upon the confocal system in the Englert Core Laboratory; they hold, as PIs, 50 NIH grants, with $9,918,884 in annual direct costs. A "major user group" has been identified. These eleven major users project 84% of the total use of the confocal system. They hold, as PIs, 20 NIH grants with $5,161, 571 in annual direct costs.

此共享仪器补助申请用于购买Leica TCS SP2激光扫描共聚焦显微镜系统。该仪器将被集成到恩格勒细胞分析实验室，这是一个共享的核心资源诺里斯棉花癌症中心在达特茅斯医学院。恩格勒实验室成立于1981年，用于流式细胞术，自1993年以来一直为达特茅斯提供成像和流式细胞仪。新的共焦仪器将取代过时的共焦系统，使用户能够获得他们所需的稳定，灵活和最新的成像功能。对新的共焦系统的要求是基于几个迫切的需求。现有的共焦系统不具有激光稳定性或激发和发射的灵活性所需的定量工作，由不同的和大量的用户。此外，有一个活细胞研究，不能满足现有的直立显微镜的立场缺乏温度控制的表达的需要。最后，一个新的系统将有能力获得12位强度分辨率的图像，将提供FRAP和反FRAP分析的兴趣区域，并将提供4个PMT的四色荧光采集-所有这些都缺乏我们目前的系统，现在需要许多用户。Leica TCS SP2系统将满足核心设施的当前用户，并将提供灵活性，以适应未来用户的需求。Englert细胞分析实验室在一名主任、一名联合主任和一个顾问委员会的控制下运作，并得到一名流程和一名成像主管的技术支持。它的财政支持来自NCI核心赠款诺里斯棉花癌症中心，每小时收费的用户，并从达特茅斯医学院的机构支持。目前，有33个NIH补助金持有人依赖于恩格勒核心实验室的共聚焦系统;他们持有，作为PI，50 NIH赠款，每年直接费用为9，918，884美元。已确定了一个“主要用户群体”。这11个主要用户占共焦系统总使用量的84%。作为PI，他们拥有20个NIH赠款，每年直接成本为5，161，571美元。