Flow Cytomety

流式细胞术/荧光成像共享资源为NCCC研究人员提供 用于细胞研究的先进仪器。为此，它的目标是提供最先进的流式细胞仪和图像分析系统以获取数据；提供计算机硬件和软件，用于分析从这些仪器获得的细胞数据；培训科学家使用这些仪器和软件；开发和实施细胞分析的新技术；并鼓励科学家就共同感兴趣的方法进行交流。 对于流式细胞仪，共享资源拥有四台Becton Dickinson仪器。带有TurboSort升级的FACStar Plus VIS/UV六参数细胞仪用于分拣和高级应用。两台FACScan五参数细胞仪和一台FACSCalibur六参数细胞仪是台式细胞仪，用于免疫表型、细胞周期分析、吞噬试验和增殖细胞前体频率分析。该资源还拥有三个图像分析系统：Bio-Rad MRC1024激光共聚焦系统、CompuCyte激光扫描细胞仪和带有冷却CCD探测器的蔡司Axiophot型光学显微镜。美国国立卫生研究院共享仪器基金刚刚收到资金，用于购买新的共焦系统。在共享资源中，有一个用于细胞准备的湿实验室和一个用于分析流动和成像数据的计算区。

The Flow Cytometry/Fluorescence Imaging Shared Resource provides NCCC investigators with advanced instrumentation for the study of cells. To this end, it aims to provide state-of-the-art flow cytometers and image analysis systems for acquisition of data; to provide computer hardware and software for the analysis of cellular data derived from these instruments; to train scientists in use of the instruments and software; to develop and implement new techniques for cell analysis; and to encourage communication among scientists on methods of common interest. For flow cytometry, the Shared Resource has four Becton Dickinson instruments. A FACStar Plus VIS/UV six-parameter cytometer with TurboSort upgrade is used for sorting and advanced applications. Two FACScan five-parameter cytometers and one FACSCalibur six-parameter cytometer are benchtop cytometers that are used for applications including immunophenotyping, cell cycle analysis, phagocytosis assays, and precursor frequency analysis of proliferating cells. The Resource also has three image analysis systems: a Bio-Rad MRC 1024 laser confocal system, a CompuCyte laser scanning cytometer, and a Zeiss Axiophot photomicroscope with a cooled CCD detector. Funding has just been received from the NIH Shared Instrumentation Grant for the purchase of a new confocal system. There is, within the Shared Resource, a wet lab for cell preparation and a computing area for analysis of flow and imaging data.