FLOW CYTOMETRY AND CELL IMAGING SHARED RESOURCE

流式细胞术和细胞成像共享资源

• 批准号: 8015001
• 负责人: Alice L. Givan
• 金额: $ 12.91万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8015001
• 关键词: 3-Dimensional; Argon; Biological Assay; Calcium; Calibration; Cancer Center; Cancer Center Support Grant; Cell Cycle; Cell physiology; Cells; Collaborations; Color; Comb animal structure; Communication; Computer software; Computers; Consult; Data; Data Analyses; Development; Documentation; Elements; Equipment; Flow Cytometry; Fluorescence; Foundations; Funding; Future; Goals; Gossypium; Grant; Hour; Image; Image Analysis; Immunophenotyping; Individual; Kinetics; Laboratories; Lasers; Leukocytes; Measures; Methods; Microscope; Mission; Monitor; National Center for Research Resources; Pattern; Preparation; Quality Control; Research; Research Personnel; Resource Sharing; Resources; Running; Scanning; Schedule; Scientist; Services; Side; Signal Transduction; Sorting - Cell Movement; Speed; Staging; Students; Surveys; System; Techniques; Technology; Temperature; Time; Tissues; Training; Work; assay development; cellular imaging; detector; digital imaging; fluorescence microscope; instrument; instrumentation; interest; medical schools; programs; reconstruction; ward

A Flow Cytometry Laboratory was established as a Shared Resource for Morris Cotton Cancer Center in 1981 with a grant from the Fannie E. Rippel Foundation for the purchase of an Ortho System 50 Cytofluorograf. That instrument was replaced in March 1991 by two Becton Dickinson flow cytometers purchased with a grant from the Shared Instrumentation Grant Program (SIG) of the National Center for Research Resources (NIH). Between 1993 and 1995, imaging capability was added to the Flow Cytometry Resource with the acquisition of a confocal imaging system with funding from the SIG program. This equipment established the Flow Cytometry and Cell Imaging Shared Resource to provide both flow and image analysis for NCCC. Since the last application for Core grant renewal, the Shared Resource has received funding from the NIH SIG program, for the purchase of a BD FACSAria sorting flow cytometer and a BD FACSCanto benchtop flow cytometer, and from Dartmouth Medical School for the purchase of a Zeiss stereo fluorescence microscope system. In addition, we have been able to acquire five Macintosh computers and have placed them in a new room to provide users with comfortable access to software for data analysis. At the present time, the Flow Cytometry and Cell Imaging Shared Resource contains four benchtop flow cytometers (two FACScans, a FACSCalibur, and a FACSCanto), two sorting flow cytometers (a FACStar Plus and a FACSAria), a Zeiss 510 Meta laser scanning confocal system, a Compucyte laser scanning cytometer, a Zeiss stereo fluorescence microscope, and a Zeiss fluorescence inverted microscope. In addition, there are six Macintosh workstations and one PC workstation for analysis of data from the flow and imaging instruments. The Flow Cytometry and Cell Imaging Shared Resource is located in a suite of seven rooms in the Borwell Research Building adjacent to the Rubin Building. It is directed by Dr. Alice L. Givan, with Dr. Paul M. Guyre as co-director. Mr. Gary Ward and Mr. Kenneth Orndorff are supervisors of the flow and imaging instruments, respectively. The Shared Resource is being used by 79 Pis, with approximately 300 users from those laboratories. It is funded by a combination of subsidy from the CCSG and chargebacks to individual users.

年建立了一个流式细胞仪实验室，作为莫里斯棉花癌中心的共享资源 1981年，由Fannie E.Rippel基金会提供赠款，用于购买Ortho System 50 赛托氟格拉夫。1991年3月，该仪器被两台Becton Dickinson Flow细胞仪取代 从国家仪器中心共享仪器资助计划(SIG)购买 研究资源(NIH)。在1993至1995年间，流式细胞术增加了成像功能 用SIG计划的资金购买共聚焦成像系统的资源。这 设备建立了流式细胞仪和细胞成像共享资源，既提供Flow，又提供 NCCC的图像分析。自上次申请核心授权续订以来，共享资源已 从NIH SIG计划获得资金，用于购买BD FACSAria分选流式细胞仪和 BD FACSCanto台式流式细胞仪，达特茅斯医学院购买蔡司 立体荧光显微镜系统。此外，我们还购买了五台Macintosh电脑 并将它们放在一个新的房间里，为用户提供舒适的访问数据分析软件的机会。 目前，流式细胞仪和细胞成像共享资源包含四个台式流程 细胞仪(两台FACScans、一台FACSCalibur和一台FACSCanto)、两台分选流式细胞仪(一台FACStar Plus和FACSAria)、蔡司510 Meta激光扫描共焦系统、Compucyte激光扫描 一台蔡司立体荧光显微镜和一台蔡司荧光倒置显微镜。在……里面 此外，还有6个Macintosh工作站和1个PC工作站，用于分析来自Flow和 成像仪器。流式细胞术和细胞成像共享资源位于一套七个 与鲁宾大楼相邻的博尔韦尔研究大楼的客房。该片由爱丽丝·L·吉万博士执导， 保罗·M·盖尔博士担任联合导演。Gary Ward先生和Kenneth Orndorff先生是该流程的主管 和成像仪器。79个PI正在使用共享资源，其中大约 来自这些实验室的300名用户。它的资金来自CCSG的补贴和按存储容量使用计费 提供给个人用户。