FLOW CYTOMETRY AND CELL IMAGING SHARED RESOURCE

年，莫里斯·科顿癌症中心建立了流式细胞术实验室，作为共享资源。 1981年，在房利美的资助下，Rippel Foundation购买Ortho System 50 细胞荧光素。该仪器于1991年3月被两台Becton Dickinson流式细胞仪取代 购买的赠款从共享仪器赠款计划（SIG）的国家中心， 研究资源（NIH）。1993年至1995年，流式细胞术增加了成像功能， 在SIG项目的资助下，获得了一个共焦成像系统。这 设备建立了流式细胞术和细胞成像共享资源， NCCC图像分析。自上一次申请核心补助金更新以来，共享资源已 从NIH SIG计划获得资金，用于购买BD FACSAria分选流式细胞仪， 一台BD FACSCanto台式流式细胞仪，以及来自达特茅斯医学院的购买蔡司 立体荧光显微镜系统。此外，我们还购买了五台麦金塔电脑， 并将它们放置在一个新的房间，为用户提供舒适的数据分析软件。 目前，流式细胞术和细胞成像共享资源包含四个台式流 流式细胞仪（两台FACScans、一台FACSCalibur和一台FACSCanto）、两台分选流式细胞仪（一台FACStar Plus和FACSAria）、Zeiss 510 Meta激光扫描共焦系统、Compucyte激光扫描共焦系统、 流式细胞仪、Zeiss立体荧光显微镜和Zeiss荧光倒置显微镜。在 此外，还有六个Macintosh工作站和一个PC工作站，用于分析流量数据， 成像仪器流式细胞术和细胞成像共享资源位于一套七个 在博韦尔研究大楼的房间毗邻鲁宾大厦。由Alice L博士主持。吉万， 保罗·M博士Guyre担任联合导演。加里沃德先生和肯尼斯奥恩多夫先生是流程的监督员 和成像仪器。共享资源由79个PI使用，大约 这些实验室的300名用户。它的资金来自CCSG的补贴和退款 个人用户。

A Flow Cytometry Laboratory was established as a Shared Resource for Morris Cotton Cancer Center in 1981 with a grant from the Fannie E. Rippel Foundation for the purchase of an Ortho System 50 Cytofluorograf. That instrument was replaced in March 1991 by two Becton Dickinson flow cytometers purchased with a grant from the Shared Instrumentation Grant Program (SIG) of the National Center for Research Resources (NIH). Between 1993 and 1995, imaging capability was added to the Flow Cytometry Resource with the acquisition of a confocal imaging system with funding from the SIG program. This equipment established the Flow Cytometry and Cell Imaging Shared Resource to provide both flow and image analysis for NCCC. Since the last application for Core grant renewal, the Shared Resource has received funding from the NIH SIG program, for the purchase of a BD FACSAria sorting flow cytometer and a BD FACSCanto benchtop flow cytometer, and from Dartmouth Medical School for the purchase of a Zeiss stereo fluorescence microscope system. In addition, we have been able to acquire five Macintosh computers and have placed them in a new room to provide users with comfortable access to software for data analysis. At the present time, the Flow Cytometry and Cell Imaging Shared Resource contains four benchtop flow cytometers (two FACScans, a FACSCalibur, and a FACSCanto), two sorting flow cytometers (a FACStar Plus and a FACSAria), a Zeiss 510 Meta laser scanning confocal system, a Compucyte laser scanning cytometer, a Zeiss stereo fluorescence microscope, and a Zeiss fluorescence inverted microscope. In addition, there are six Macintosh workstations and one PC workstation for analysis of data from the flow and imaging instruments. The Flow Cytometry and Cell Imaging Shared Resource is located in a suite of seven rooms in the Borwell Research Building adjacent to the Rubin Building. It is directed by Dr. Alice L. Givan, with Dr. Paul M. Guyre as co-director. Mr. Gary Ward and Mr. Kenneth Orndorff are supervisors of the flow and imaging instruments, respectively. The Shared Resource is being used by 79 Pis, with approximately 300 users from those laboratories. It is funded by a combination of subsidy from the CCSG and chargebacks to individual users.