PURCHASE OF A 6 PARAMETER BENCH TOP FLOW CYTOMETER

购买 6 参数台式流式细胞仪

• 批准号: 2791066
• 负责人: Alice L. Givan
• 金额: $ 13.29万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791066
• 关键词: biomedical equipment; biomedical equipment purchase; cell sorting; flow cytometry

This Shared Instrument Grant application is for funding for the purchase of a Becton Dickinson FACSCalibur bench-top flow cytometer. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a Shared Resource of the Norris Cotton Cancer Center and which has been providing Dartmouth College and Medical School with flow cytometry instrumentation since 1981. The new instrument will join a FACScan bench-top flow cytometer, a FACStar Plus cell sorter, and three imaging systems in a suite of rooms in the Borwell Research Building at the Dartmouth-Hitchcock Medical Center. The need for an additional bench-top flow cytometer is base don two urgent requirements: First, the existing FACScan cytometer is heavily booked and his no longer able to provide the time and flexibility necessary for efficient research. Second, there is an expressed need that cannot be met on the existing instrumentation for four-color analysis and for the ability to use far-red excitable flurochromes. Because it has recognized the urgent requirement for the purchase of an additional flow cytometer, the Research Committee of Dartmouth Medical School has confirmed a contribution of $10,000 toward the purchase of this instrument. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and an imaging supervisor. A "major user group" has been identified, consisting of eleven NIH-funded Principal Investigators dependent on the use of the flow cytometers in the Englert Laboratory for their research. This group of investigators has been responsible for 92% of the use of the FACScan cytometer in 1997 and predicts increased requirements for a bench-top cytometer in the future. They currently hold 24 NIH-funded research grants totalling $5,109,299 in annual direct costs.

该共享仪器拨款申请旨在资助购买 Becton Dickinson FACSCalibur 台式流式细胞仪。该仪器将集成到 Englert 细胞分析实验室中，该实验室是 Norris Cotton 癌症中心的共享资源，自 1981 年以来一直为达特茅斯学院和医学院提供流式细胞仪。新仪器将与达特茅斯-希区柯克医学中心 Borwell 研究大楼一套房间中的 FACScan 台式流式细胞仪、FACStar Plus 细胞分选仪和三个成像系统一起使用。需要一台额外的台式流式细胞仪是基于两个紧迫的需求：首先，现有的 FACScan 细胞仪已被大量预订，不再能够提供高效研究所需的时间和灵活性。其次，现有的四色分析仪器和使用远红可激发荧光染料的能力无法满足明确的需求。由于认识到购买额外一台流式细胞仪的迫切需要，达特茅斯医学院研究委员会已确认捐赠 10,000 美元购买该仪器。恩格勒特细胞分析实验室在一名主任、一名联合主任和一个顾问委员会的控制下运作，并得到流程和成像主管的技术支持。已经确定了一个“主要用户组”，由 11 名 NIH 资助的主要研究人员组成，他们的研究依赖于 Englert 实验室流式细胞仪的使用。这组研究人员负责 1997 年 FACScan 细胞仪 92% 的使用，并预测未来对台式细胞仪的需求将会增加。他们目前持有 24 项 NIH 资助的研究经费，年度直接费用总计 5,109,299 美元。