PURCHASE OF A 6 PARAMETER BENCH TOP FLOW CYTOMETER

这项共享仪器赠款申请是为购买Becton Dickinson FACSCalibur台式流式细胞仪提供资金。该仪器将被整合到恩格勒特细胞分析实验室，该实验室是诺里斯棉花癌症中心的共享资源，自1981年以来一直为达特茅斯学院和医学院提供流式细胞仪仪器。这台新仪器将与FACScan台式流式细胞仪、FACStar Plus细胞分选仪和三个成像系统一起放在达特茅斯-希区柯克医学中心Borwell研究大楼的一套房间里。需要额外的台式流式细胞仪是基于两个迫切的要求：首先，现有的FACScan细胞仪被大量预订，HIS不再能够提供有效研究所需的时间和灵活性。其次，现有的四色分析仪器和使用远红可激发荧光染料的能力无法满足这一需求。由于达特茅斯医学院的研究委员会已经认识到购买一台额外的流式细胞仪的迫切需求，它已经确认为购买这台仪器捐款1万美元。恩格勒特细胞分析实验室在一名主任、一名联席主任和一个咨询委员会的控制下运作，并在流程和成像主管的技术支持下运行。已经确定了一个“主要用户组”，由11名由美国国立卫生研究院资助的首席研究人员组成，他们依赖恩格勒实验室的流式细胞仪进行研究。这组研究人员负责了1997年FACScan细胞仪92%的使用，并预测未来对台式细胞仪的需求将会增加。他们目前拥有24笔由美国国立卫生研究院资助的研究拨款，每年的直接成本总计为5109,299美元。

This Shared Instrument Grant application is for funding for the purchase of a Becton Dickinson FACSCalibur bench-top flow cytometer. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a Shared Resource of the Norris Cotton Cancer Center and which has been providing Dartmouth College and Medical School with flow cytometry instrumentation since 1981. The new instrument will join a FACScan bench-top flow cytometer, a FACStar Plus cell sorter, and three imaging systems in a suite of rooms in the Borwell Research Building at the Dartmouth-Hitchcock Medical Center. The need for an additional bench-top flow cytometer is base don two urgent requirements: First, the existing FACScan cytometer is heavily booked and his no longer able to provide the time and flexibility necessary for efficient research. Second, there is an expressed need that cannot be met on the existing instrumentation for four-color analysis and for the ability to use far-red excitable flurochromes. Because it has recognized the urgent requirement for the purchase of an additional flow cytometer, the Research Committee of Dartmouth Medical School has confirmed a contribution of $10,000 toward the purchase of this instrument. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and an imaging supervisor. A "major user group" has been identified, consisting of eleven NIH-funded Principal Investigators dependent on the use of the flow cytometers in the Englert Laboratory for their research. This group of investigators has been responsible for 92% of the use of the FACScan cytometer in 1997 and predicts increased requirements for a bench-top cytometer in the future. They currently hold 24 NIH-funded research grants totalling $5,109,299 in annual direct costs.