THREE-DIMENSIONAL ELECTRON MICROSCOPY OF MACROMOLECULES

大分子三维电子显微镜

• 批准号: 2893518
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 0.5万
• 依托单位: GORDON RESEARCH CONFERENCES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-15 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2893518
• 关键词: bioimaging /biomedical imaging; electron microscopy; meeting /conference /symposium; structural biology; travel

DESCRIPTION (Adapted from Applicant's Abstract) This proposal request support for the 8th Gordon Research Conference on 3-Dimensional Electron Microscopy of Macromolecules. Electron microscopy occupies a unique position in the biological armamentarium in that it can address questions at the molecular level that can not be approached using other structural methods. In recent years, important technical breakthroughs have been made that enable 3-D structures to be determined with ever increasing accuracy, and which have opened the way to the study of macromolecular systems under defined physiological conditions. As a result, there are exciting new opportunities for understanding biological mechanisms, interactions between macromolecules and the principles of biomolecular design. This series of conferences fosters an integrated view of biological complexity by bringing together scientists working at different levels of structural hierarchy. These meetings serve to define the challenges of the future and to focus a concerted approach to problems in the preparation, imaging and interpretation of biological specimens that range in size from the molecular to the subcellular. The impact of the preceding seven conferences has been such that the 3-D EM community has come to regard this Gordon Conference as an event of pivotal importance. The theme of the conference is "New Challenges for Three Dimensional Electron Microscopy". In organizing this conference, our goal has been to create a dynamic forum for the dissemination of the major progress in the field over the past two years, and identification of the new technical and biological challenges that the field must address in order for 3-D EM to become an essential and ubiquitous tool for the solution of cell biological problems. In the spirit of the Gordon Conference, the applicants have chosen a program that represents the frontier of the field. Speakers have been chosen on the basis of the importance of their recent contributions and the potential impact of their particular research approach in future work in this field. Based on past experience, the applicants confidently expect that the 3-D EM Gordon Conference will continue to play a central role in defining new technical challenges, and in focusing a concerted approach to the understanding of fundamental biological questions.

本提案请求对第八届戈登研究会议大分子三维电子显微镜的支持。电子显微镜在生物仪器中占有独特的地位，因为它可以解决使用其他结构方法无法解决的分子水平问题。近年来，已经取得了重要的技术突破，使得能够以越来越高的精度确定3D结构，并且为在限定的生理条件下研究大分子系统开辟了道路。因此，有令人兴奋的新的机会，了解生物机制，大分子之间的相互作用和生物分子设计的原则。这一系列会议通过汇集在不同结构层次上工作的科学家，促进了对生物复杂性的综合看法。这些会议有助于确定未来的挑战，并集中协调一致的方法来解决从分子到亚细胞大小不等的生物标本的制备、成像和解释方面的问题。前七次会议的影响已经使得3D EM社区已经将这次戈登会议视为具有关键重要性的事件。会议的主题是“三维电子显微镜的新挑战”。在组织这次会议，我们的目标是创建一个动态的论坛，传播该领域的主要进展，在过去的两年中，并确定新的技术和生物学挑战，该领域必须解决，以使3-D EM成为一个必不可少的和无处不在的工具，为解决细胞生物学问题。本着戈登会议的精神，申请人选择了一个代表该领域前沿的项目。发言者的选择是根据他们最近的贡献的重要性和他们的特定研究方法在这一领域的未来工作的潜在影响。根据过去的经验，申请人充满信心地期望，3-D EM戈登会议将继续在定义新的技术挑战方面发挥核心作用，并集中精力采取协调一致的方法来理解基本的生物学问题。