PARAVENTRICULAR NUCLEUS/AFFERENT PEPTIDERGIC SYNAPTOLOGY

室旁核/传入肽联会

• 批准号: 3074748
• 负责人: DIANE T PIEKUT
• 金额: $ 4.96万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3074748
• 关键词: adrenocorticotropic hormone; autonomic nervous system; electron microscopy; histochemistry /cytochemistry; homeostasis; hypothalamus; immunochemistry; microscopy; neuroanatomy; neuroendocrine system; oxytocin; somatostatin; synapses; vasopressins; visceral afferent nerve

The paraventricular nucleus (PVN) of hypothalamus is emerging as a central site for the integration and coordination of some neuroendocrine and autonomic visceral responses and its importance in the maintenance of homeostasis is proposed. Efferent fiber projections of PV neurons to the pituitary gland and specific brainstem centers associated with the control of cardiovascular and respiratory functions are known. The studies proposed will further our understanding of the organization of afferent peptidergic fiber projections to the paraventricular nucleus and elucidate the significance of these pathways in relation to the neuroendocrine and autonomic functions of the PVN. The specific aims of this proposal are (1) to describe the cytoarchitectonic organization of ACTH/Beta-endorphin, enkephalin and somatostatin immunoreactive neurons within the functionally distinct subnuclei of the PVN complex and (2) to elucidate the intrinsic synaptic organization and potential interactions and communications of these three central peptidergic systems and magnocellular (vasopressin/oxytocin) PV neurons. The studies proposed will provide an anatomical substrate for functional interactions described between each of the selected neuropeptidergic systems and magnocellular neurons to occur. These findings will be of considerable importance to the neuroendocrinologist interested in the control mechanism of vasopressin and oxytocin secretion and their effects on neuroendocrine and autonomic visceral (e.g. cardiovascular) functions. Anatomical details will be elucidated using Vibratome tissue sections and the basic and double antigen immunocytochemical procedures at both the light and electron microscopic levels.

下丘脑室旁核(PVN)是一种中枢神经系统 一些神经内分泌和协调的整合和协调的场所 自主神经内脏反应及其在维持心脏功能中的重要性 提出了动态平衡的概念。PV神经元向下丘脑的传出纤维投射 与对照相关的脑垂体腺和特定脑干中心 心血管和呼吸功能的影响是已知的。这些研究 提出将进一步加深我们对传入组织的理解 肽能纤维向室旁核的投射及其意义 这些通路与神经内分泌和 室旁核的自主神经功能。这项建议的具体目的是(1) 为了描述ACTH/β-内啡肽的细胞结构组织， 脑啡肽和生长抑素免疫反应神经元内的功能 PVN复合体的不同亚核和(2)阐明本征 突触的组织和潜在的相互作用和交流 这三个中枢肽能系统与巨噬细胞 (加压素/催产素)PV神经元。 所提出的研究将为功能 描述了所选择的每个神经肽能之间的相互作用 系统和大细胞神经元的出现。这些发现将是 对感兴趣的神经内分泌学家来说相当重要 加压素和催产素分泌的调控机制及其作用 神经内分泌和自主内脏(如心血管)功能。 解剖细节将使用可控震颤器组织切片和 基本和双重抗原免疫细胞化学程序在两个 光镜和电子显微镜水平。