PARAVENTRICULAR NUCLEUS: PEPTIDE COEXISTENCE/SYNAPTOLOGY

室旁核：肽共存/联会学

• 批准号: 3398632
• 负责人: DIANE T PIEKUT
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3398632
• 关键词: adrenocorticotropic hormone; autonomic nervous system; electron microscopy; endorphins; enkephalins; histochemistry /cytochemistry; hormone regulation /control mechanism; immunochemistry; laboratory rat; microscopy; neural information processing; neuroanatomy; neuroendocrine system; neuropeptides; oxytocin; paraventricular nucleus; somatostatin; synapses; vasopressins; visceral afferent nerve

The paraventricular nucleus (PVN) of hypothalamus is emerging as a central site for the integration and coordination of some neuroendocrine and autonomic visceral responses and its importance in the maintenance of homoeostasis is proposed. Efferent fiber projections of PV neurons to the pituitary gland and specific brainstem centers associated with the control of cardiovascular and respiratory functions are known. The studies proposed will further our understanding of the organization of afferent peptidergic fiber projections to the paraventricular nucleus and elucidate the significance of these pathways in relation to the neuroendocrine and autonomic functions of the PVN. The specific aims of this proposal are (1) to describe the cytoarchitectonic organization of ACTH/Beta-endorphin, enkephalin and somatostatin immunoreactive neurons within the functionally distinct subnuclei of the PVN complex and (2) to elucidate the instrinsic synaptic organization and potential interactions and communications of these three central peptidergic systems and magnocellular (vasopressin/oxytocin) PV neurons. The studies proposed will provide an anatomical substrate for functional interactions described between each of the selected neuropeptidergic systems and magnocellular neurons to occur. These findings will be of considerable importance to the neuroendocrinologist interested in the control mechanism of vasopressin and oxytocin secretion and their effects on neuroendocrine and autonomic visceral (e.g. cardiovascular) functions. Anatomical details will be elucidated using Vibratome tissue sections and the basic and double antigen immunocytochemical procedures at both the light and electron microscopic levels.

下丘脑室旁核（PVN）正在成为中枢神经系统 整合和协调某些神经内分泌和 自主内脏反应及其在维持 提出了稳态。 PV神经元的传出纤维投射到 脑下垂体和特定的脑干中枢与控制 心血管和呼吸功能的影响是已知的。 研究 提出将进一步我们的组织传入的理解 肽能纤维投射到室旁核，并阐明 这些通路与神经内分泌和 PVN的自主功能。 本建议的具体目标是：（1） 为了描述ACTH/β-内啡肽的细胞结构组织， 脑啡肽和生长抑素免疫反应神经元的功能 PVN复合体的不同亚核和（2）阐明了PVN复合体的内在结构， 突触组织和潜在的相互作用和通信 这三个中心肽能系统和巨细胞 （加压素/催产素）PV神经元。 提出的研究将提供一个解剖学基础的功能 所述的每种选定的神经肽能之间的相互作用 系统和大细胞神经元的发生。 这些发现将是 对于神经内分泌学家来说， 加压素和催产素分泌的调控机制及其作用 对神经内分泌和自主内脏（如心血管）功能的影响。 解剖学细节将使用振动切片机组织切片进行阐明， 基础和双抗原免疫细胞化学程序在两个 光学和电子显微镜水平。