How does targeted proteolysis regulate ABA signalling?

靶向蛋白水解如何调节 ABA 信号传导？

• 批准号: BB/G010595/1
• 负责人: Michael Holdsworth
• 金额: $ 57.83万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG010595%2F1
• 关键词: How; does; targeted; proteolysis; regulate

Through our genetic and transcriptomic analyses we have shown that the N-end rule pathway is key to removing ABA sensitivity for germination, that it does this by interacting with the ABA transduction pathway, and that it influences the transcriptome by relieving the ABA-associated repression of a cohort of genes through removal of an ABA signal transduction-related protein substrate(s). Therefore our identification of the role of this protein degradation pathway offers a timely and unique window of opportunity to understand how targeted proteolysis regulates ABA signalling. The key hypotheses that need addressing are: 1. That germination induced endo-peptidase activity initiates the degradation of a key component(s) of ABA signalling through the N-end rule pathway. 2. That the ABA-related transcription factor ABI3 represents a substrate for the N-end rule pathway. 3. That the endosperm is the major site of action of the N-end rule pathway in the control of germination potential. 4. That the N-end rule pathway influences germination potential through a genetic network of mutually-exclusive repression and activation of key enzymes of endosperm functionality, via the transcription factors ABI3 and EIL4. 5. That the N-end rule pathway may have other functions during plant growth and development associated with removal of ABA sensitivity.

通过我们的遗传和转录组分析，我们已经证明N-端规则通路是消除脱落酸对萌发的敏感性的关键，它通过与脱落酸转导途径相互作用来实现这一点，并且它通过去除脱落酸信号转导相关蛋白底物(S)来解除脱落酸相关的一系列基因的抑制而影响转录组。因此，我们对这一蛋白质降解途径作用的识别提供了一个及时而独特的机会之窗，以了解靶向蛋白分解如何调节ABA信号。需要解决的关键假说是：1.萌发诱导的内肽酶活性通过N-端规则途径启动脱落酸信号的关键成分(S)的降解。2.ABA相关转录因子ABI3是N-端规则途径的底物。3.胚乳是N-末端规则通路控制发芽势的主要作用部位。4.N-末端规则途径通过转录因子ABI3和EIL4通过相互排斥的抑制和激活胚乳功能关键酶的遗传网络来影响发芽势。5.N-末端调节通路在植物生长发育过程中可能还具有与ABA敏感性去除相关的其他功能。

项目成果

Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6.

• DOI: 10.1111/pbi.12334
• 发表时间: 2016-01
• 期刊: Plant biotechnology journal
• 影响因子: 13.8
• 作者: Mendiondo GM;Gibbs DJ;Szurman-Zubrzycka M;Korn A;Marquez J;Szarejko I;Maluszynski M;King J;Axcell B;Smart K;Corbineau F;Holdsworth MJ
• 通讯作者: Holdsworth MJ

Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants.

• DOI: 10.1038/nature10534
• 发表时间: 2011-10-23
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Gibbs, Daniel J.;Lee, Seung Cho;Isa, Nurulhikma Md;Gramuglia, Silvia;Fukao, Takeshi;Bassel, George W.;Correia, Cristina Sousa;Corbineau, Francoise;Theodoulou, Frederica L.;Bailey-Serres, Julia;Holdsworth, Michael J.
• 通讯作者: Holdsworth, Michael J.

Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors.

• DOI: 10.1016/j.molcel.2013.12.020
• 发表时间: 2014-02-06
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Gibbs, Daniel J.;Isa, Nurulhikma Md;Movahedi, Mahsa;Lozano-Juste, Jorge;Mendiondo, Guillermina M.;Berckhan, Sophie;Marin-de la Rosa, Nora;Conde, Jorge Vicente;Correia, Cristina Sousa;Pearce, Simon P.;Bassel, George W.;Hamali, Bulut;Talloji, Prabhavathi;Tome, Daniel F. A.;Coego, Alberto;Beynon, Jim;Alabadi, David;Bachmair, Andreas;Leon, Jose;Gray, Julie E.;Theodoulou, Frederica L.;Holdsworth, Michael J.
• 通讯作者: Holdsworth, Michael J.

Oxygen sensing coordinates photomorphogenesis to facilitate seedling survival.

• DOI: 10.1016/j.cub.2015.03.060
• 发表时间: 2015-06-01
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Abbas, Mohamad;Berckhan, Sophie;Rooney, Daniel J.;Gibbs, Daniel J.;Conde, Jorge Vicente;Correia, Cristina Sousa;Bassel, George W.;Marin-de la Rosa, Nora;Leon, Jose;Alabadi, David;Blazquez, Miguel A.;Holdsworth, Michael J.
• 通讯作者: Holdsworth, Michael J.