The N-end rule pathway controls plant response to drought

N端规则途径控制植物对干旱的反应

• 批准号: BB/K000144/1
• 负责人: Michael Holdsworth
• 金额: $ 44.54万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK000144%2F1
• 关键词: end; rule; pathway; controls; plant

In order to maintain food security for a growing population under climate change, there is a pressing need to develop crops that use less water and that are more tolerant to environmental stresses, particularly drought. Our recent research at the Universities of Nottingham and Sheffield using the laboratory model Arabidopsis thaliana (the plant equivalent of a lab-rat) has identified plants that are extremely tolerant to drought stress. The drought tolerant plants lack the ability to recognise particular proteins inside their cells and send them for destruction by a biochemical process called the N-end rule pathway. From our analysis so far, we already have an important clue as to the identity of the 'drought tolerance protein' that is not destroyed and is therefore stabilised in our drought-tolerant plants. We know that this protein has the amino acid residues methionine and cysteine at one end. In this project we shall identify which specific protein(s) are stabilised in our plants providing us with important information on how we could, in the future, make plants more drought-tolerant. We will also find out if the same N-end rule drought tolerance system works in an important UK crop, barley. Many parts of the UK have experienced unusual and extended periods of drought over the past year which has included the driest 12 months since records began in 1910. This has led to a high level of crop failure with 2011 grain yields being particularly affected. Farmers have reported failure of 10% to 50% of their barley crop, and the surviving barley grain has often been of poor quality, only suitable for low value animal feed rather than for beer-making. As this is a problem for both farmers and brewers, SABMiller a major UK brewing company have agreed to fund part of our research project. SABMiller are committed to reducing the amount of water that they use, and believe that a better understanding of how barley responds to drought may help them to achieve this aim.Our research falls directly within the remit of Global Food Security and Living with Environment Change cross-council priorities identified by the UK Research Councils. The project will help to address the BBSRC strategic research priority Food Security (Crop Science) and our collaboration with SABMiller addresses the Building Partnerships (Collaborative Research with Users) agenda.

为了在气候变化的情况下为不断增长的人口维持粮食安全，迫切需要培育用水量较少、更能承受环境压力、特别是干旱的作物。我们最近在诺丁汉大学和谢菲尔德大学使用实验室模型拟南芥（相当于实验室大鼠的植物）进行的研究已经确定了对干旱胁迫具有极强耐受性的植物。耐旱植物缺乏识别细胞内特定蛋白质的能力，并通过称为N端规则途径的生化过程将其破坏。从我们到目前为止的分析，我们已经有了一个重要的线索，关于“耐旱蛋白”的身份，它没有被破坏，因此在我们的耐旱植物中是稳定的。我们知道，这种蛋白质的氨基酸残基甲硫氨酸和半胱氨酸在一端。在这个项目中，我们将确定哪些特定的蛋白质在我们的植物中是稳定的，为我们提供关于未来如何使植物更耐旱的重要信息。我们还将发现相同的N端规则耐旱系统是否适用于英国重要的作物大麦。在过去的一年里，英国许多地区经历了不寻常的长期干旱，其中包括自1910年有记录以来最干旱的12个月。这导致作物严重歉收，2011年的粮食产量受到的影响尤其严重。农民们报告说，他们的大麦作物有10%到50%歉收，幸存的大麦籽粒往往质量差，只适合作为低价值的动物饲料，而不适合酿造啤酒。由于这对农民和酿酒商都是一个问题，英国一家主要的酿酒公司SABMiller同意资助我们的部分研究项目。SABMiller致力于减少用水量，并相信更好地了解大麦如何应对干旱可能有助于他们实现这一目标。我们的研究福尔斯直接属于英国研究理事会确定的全球粮食安全和与环境变化共存的跨理事会优先事项的职权范围。该项目将有助于解决BBSRC战略研究优先粮食安全（作物科学），我们与SABMiller的合作解决了建立伙伴关系（与用户的合作研究）议程。

项目成果

Additional file 4: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

附加文件 4：受芸苔根肿菌感染的拟南芥根部缺氧反应支持根肿病的发育

• DOI: 10.6084/m9.figshare.c.3599162_d4
• 发表时间: 2016
• 作者: Gravot A

Additional file 2: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

附加文件2：受芸苔根肿菌感染的拟南芥根部的缺氧反应支持根肿病的发育

• DOI: 10.6084/m9.figshare.c.3599162_d2
• 发表时间: 2016
• 作者: Gravot A

Oxygen sensing coordinates photomorphogenesis to facilitate seedling survival.

• DOI: 10.1016/j.cub.2015.03.060
• 发表时间: 2015-06-01
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Abbas, Mohamad;Berckhan, Sophie;Rooney, Daniel J.;Gibbs, Daniel J.;Conde, Jorge Vicente;Correia, Cristina Sousa;Bassel, George W.;Marin-de la Rosa, Nora;Leon, Jose;Alabadi, David;Blazquez, Miguel A.;Holdsworth, Michael J.
• 通讯作者: Holdsworth, Michael J.

Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors.

• DOI: 10.1016/j.molcel.2013.12.020
• 发表时间: 2014-02-06
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Gibbs, Daniel J.;Isa, Nurulhikma Md;Movahedi, Mahsa;Lozano-Juste, Jorge;Mendiondo, Guillermina M.;Berckhan, Sophie;Marin-de la Rosa, Nora;Conde, Jorge Vicente;Correia, Cristina Sousa;Pearce, Simon P.;Bassel, George W.;Hamali, Bulut;Talloji, Prabhavathi;Tome, Daniel F. A.;Coego, Alberto;Beynon, Jim;Alabadi, David;Bachmair, Andreas;Leon, Jose;Gray, Julie E.;Theodoulou, Frederica L.;Holdsworth, Michael J.
• 通讯作者: Holdsworth, Michael J.

Additional file 1: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

附加文件 1：受芸苔根肿菌感染的拟南芥根部缺氧反应支持根肿病的发育

• DOI: 10.6084/m9.figshare.c.3599162_d3
• 发表时间: 2016
• 作者: Gravot A