REGULATION OF MICROBIAL NITROGEN METABOLISM

微生物氮代谢的调节

• 批准号: 3126444
• 负责人: ROBERT A BENDER
• 金额: $ 16.86万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3126444
• 关键词: bacterial genetics; chromatography; cyclic AMP; enzyme mechanism; enzyme structure; gel electrophoresis; gene expression; genetic mapping; genetic promoter element; genetic transcription; glutamate ammonia ligase; microorganism genetics; microorganism metabolism; nitrogen metabolism; operon; protein sequence; temperature sensitive mutant

The genes for Histidine UTilization (hut) in Klebsiella aerogenes are typical of a large number of genes whose expression is strictly regulated by the available supply of nitrogen. Thus nitrogen regulation of hut serves as a model system for this kind of pleiotropic control. The goal of the research proposed here is to develop an in vitro transcription system for the hut operon of K. aerogenes that is responsive to regulation by the nitrogen regulatory factor(s) and to study the molecular basis for that regulation. The specific objectives of the five-year proposal period are: (1) To determine the mechanism of carbon regulation at the promoter of the hutUH operon, (2) To begin an analysis of the nitrogen regulation at the promoter of the hutUH operon, and (3) To extend our understanding of the hut operons themselves.

产气克雷伯菌组氨酸利用基因（hut）为