REGULATION OF MICROBIAL NITROGEN METABOLISM
微生物氮代谢的调节
基本信息
- 批准号:3126442
- 负责人:
- 金额:$ 16.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1979
- 资助国家:美国
- 起止时间:1979-05-01 至 1991-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The genes for Histidine UTilization (hut) in Klebsiella aerogenes are
typical of a large number of genes whose expression is strictly regulated
by the available supply of nitrogen. Thus nitrogen regulation of hut
serves as a model system for this kind of pleiotropic control. The goal of
the research proposed here is to develop an in vitro transcription system
for the hut operon of K. aerogenes that is responsive to regulation by the
nitrogen regulatory factor(s) and to study the molecular basis for that
regulation. The specific objectives of the five-year proposal period are:
(1) To determine the mechanism of carbon regulation at the promoter of the
hutUH operon, (2) To begin an analysis of the nitrogen regulation at the
promoter of the hutUH operon, and (3) To extend our understanding of the
hut operons themselves.
产气克雷伯氏菌的组氨酸利用(HUT)基因是
典型的大量表达受到严格调控的基因
由可用的氮气供应决定。因此,小屋的氮素调控
作为这种多效控制的模型系统。的目标是
本研究旨在开发一种体外转录系统。
对于产气克雷伯氏菌的小屋操纵子来说,它对
氮调节因子(S)及其分子基础研究
监管。五年建议期的具体目标是:
(1)确定碳调节机制的启动子
HutUH操纵子,(2)开始分析
HUH操纵子的推动者,以及(3)扩大我们对
小屋本身就在运作。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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