PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOCYTES

这个项目的目的是定义人类遗传基础 单核巨噬细胞增殖、分化的调控 和功能异构性。此外，我们希望定义 造血生长因子在其调控中的作用 从多能的造血干细胞中产生。我们 将通过使用当前可用的 对分化抗原的抗体进行鉴定和分类，使用 流式细胞仪检测，细胞处于独特的分化阶段。通过 在选定的存在的情况下培养这些分类的细胞 造血生长因子IL-3、GM-CSF和M-CSF，我们将 测定其分化动力学。这将是 通过使用一组或单抗来定义 作为时间函数出现的后代的表型 从已分类的前体中分离出来。 我们还将研究原癌基因的表达。 使用目前可用的抗体的产品。此表达式将 与细胞的增殖阶段有关，以及 它们的分化阶段。通过这种方式，我们将能够 确定该基因的表达是否与细胞周期有关 与分化无关，其表达是否与 差异化或两者兼而有之。 这些研究的结果将对我们的 了解正常细胞的生产和功能是如何 在健康和疾病方面得到控制。

The object of this project is to define the genetic basis for the regulation of mononuclear phagocyte proliferation, differentiation and functional heterogeneity. In addition, we wish to define the role hematopoietic growth factors play in the control of their production from the multipotent hematopoietic stem cell. We will approach this objective by using currently available antibodies to differentiation antigens to identify and sort, using flow cytometry, cells at unique differentiation stages. By culturing these sorted cells in the presence of the selected hematopoietic growth factors IL3, GM-CSF and M-CSF, we will determine their differentiation kinetics. This will be accomplished by using a panel or monoclonal antibodies to define the phenotype of the progeny which emerge as a function of time from the sorted precursors. We will also investigate the expression of proto-oncogene products using currently available antibodies. This expression will be correlated with the proliferation stages of the cells as well as their differentiation stage. In this way, we will be able to determine if the gene's expression is cell cycle related and independent of differentiation, whether its expression is related to differentiation or both. Results of these studies will have an enormous impact on our understanding of how a normal cell's production and function is controlled in health and disease.