PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOCYTES

这个项目的目的是确定遗传基础的 单核吞噬细胞增殖、分化的调节 和功能异质性。 此外，我们希望定义 造血生长因子在控制 从多能造血干细胞产生。 我们 将利用现有的 分化抗原的抗体来识别和分类，使用 流式细胞术，细胞在独特的分化阶段。 通过 在所选择的细胞存在下培养这些分选的细胞， 造血生长因子IL 3，GM-CSF和M-CSF，我们将 确定它们的分化动力学。 这将是 通过使用面板或单克隆抗体来定义 作为时间函数出现的后代表型 从已分类的前体中。 我们还将研究原癌基因的表达 使用现有抗体的产品。 这个表达式将 与细胞的增殖阶段相关， 的分化阶段。 这样，我们就能够 确定基因的表达是否与细胞周期相关， 与分化无关，其表达是否相关 差异化或两者兼而有之。 这些研究的结果将对我们的 了解正常细胞的生产和功能是如何 控制健康和疾病。

The object of this project is to define the genetic basis for the regulation of mononuclear phagocyte proliferation, differentiation and functional heterogeneity. In addition, we wish to define the role hematopoietic growth factors play in the control of their production from the multipotent hematopoietic stem cell. We will approach this objective by using currently available antibodies to differentiation antigens to identify and sort, using flow cytometry, cells at unique differentiation stages. By culturing these sorted cells in the presence of the selected hematopoietic growth factors IL3, GM-CSF and M-CSF, we will determine their differentiation kinetics. This will be accomplished by using a panel or monoclonal antibodies to define the phenotype of the progeny which emerge as a function of time from the sorted precursors. We will also investigate the expression of proto-oncogene products using currently available antibodies. This expression will be correlated with the proliferation stages of the cells as well as their differentiation stage. In this way, we will be able to determine if the gene's expression is cell cycle related and independent of differentiation, whether its expression is related to differentiation or both. Results of these studies will have an enormous impact on our understanding of how a normal cell's production and function is controlled in health and disease.