课题基金 / 基金详情

MOLECULAR PHENOTYPING BY FLOW CYTOMETRY

MOLECULAR PHENOTYPING BY FLOW CYTOMETRY
通过流式细胞术进行分子表型分析
批准号:
3203941
负责人:
CARLETON C STEWART
金额:
$7.72万
依托单位国家:
美国
项目类别:
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-09-23 至 1995-07-31

项目摘要

项目成果

CARLETON C STEWART的其他基金

相关文献

中文摘要
翻译
分子生物学研究将发展分子生物学的研究方法
英文摘要
Molecular Biology Research will develop methods for molecular phenotyping of cancer cells by flow cytometry. Three specific aims are proposed: Aim one is to isolate cell populations of interest by cell sorting and determine gene amplification levels by PCR on less than 1000 cells (not the millions of cells required for standard blotting techniques). Aims two and three are to develop two new technologies, fluorescence in-situ hybridization on suspension (FISHES (aim 2) and fluorescence in-situ polymerase chain reaction (FLIP) (aim 3). The goal of these studies is to correlate gene expression at the mRNA level by FISHES or FLIP with protein expression by immunofluorescence.
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会议论文
A Shared Multiparameter Flow Cytometer and Cell Sorter
MOLECULAR PHENOTYPING BY FLOW CYTOMETRYI
  • 批准号:
    3203940
  • 项目类别:
  • 资助金额:
    $6.82万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位:
MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF SOLID TUMORS
  • 批准号:
    3203942
  • 项目类别:
  • 资助金额:
    $7.23万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位:
MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF SOLID TUMORS
  • 批准号:
    2100899
  • 项目类别:
  • 资助金额:
    $8.45万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位: