MOLECULAR PHENOTYPING BY FLOW CYTOMETRY

通过流式细胞术进行分子表型分析

• 批准号: 3203941
• 负责人: CARLETON C STEWART
• 金额: $ 7.72万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-23 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3203941
• 关键词: cell population study; diagnosis design /evaluation; flow cytometry; gene expression; human tissue; immunofluorescence technique; in situ hybridization; molecular oncology; natural gene amplification; neoplasm /cancer diagnosis; neoplasm /cancer genetics; phenotype; polymerase chain reaction

Molecular Biology Research will develop methods for molecular phenotyping of cancer cells by flow cytometry. Three specific aims are proposed: Aim one is to isolate cell populations of interest by cell sorting and determine gene amplification levels by PCR on less than 1000 cells (not the millions of cells required for standard blotting techniques). Aims two and three are to develop two new technologies, fluorescence in-situ hybridization on suspension (FISHES (aim 2) and fluorescence in-situ polymerase chain reaction (FLIP) (aim 3). The goal of these studies is to correlate gene expression at the mRNA level by FISHES or FLIP with protein expression by immunofluorescence.

分子生物学研究将发展分子生物学方法 用流式细胞术对癌细胞进行表型分析。三个具体目标是 建议：目标一是以细胞为单位分离感兴趣的细胞群 用聚合酶链式反应对1000个以下个体进行基因分选和基因扩增水平测定 细胞(不是标准印迹所需的数百万个细胞 技术)。目标二和三是开发两项新技术， 悬浮液上的荧光原位杂交(鱼类(目标2)和 荧光原位聚合酶链式反应(FliP)(目的3)。目标是 这些研究的目的是通过以下方式在mRNA水平上关联基因表达 鱼或翻转蛋白的免疫荧光表达。