PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOGYTES

小鼠和人单核巨噬细胞的增殖动力学 被研究。和所需因素之间的关系 促进扩散和抑制扩散的将是 已定义。我们相信，一旦我们了解了核扩散是如何 我们将能够在疾病期间改变宿主的环境 各国动员有效数量的单核巨噬细胞。没有闲聊 我们试图在多大程度上减轻病原体或肿瘤的负担 这些正常的宿主防御细胞所对抗的负担，它们将是 如果可用资源太少，则无效。我们也在研究 单核巨噬细胞的增殖，以提供这些细胞的克隆 足够多的细胞来研究它们的功能异质性。 利用流式细胞仪和细胞分选仪，我们将研究分化 当单核巨噬细胞增殖时。通过去除增长因素 我们也可以在没有增殖的情况下研究它们的分化。 我们计划研究以下分化标志： 非贴壁骨髓祖细胞来源的子代的黏附， 巨噬细胞吞噬功能的发展及膜标记物的研究进展 IA、Fc受体和C3b受体。我们将能够从以下方面确定 这些研究无论是功能或标记表达的单一明确 吞噬细胞代表分化的状态，所有细胞都通过或 某些函数是否是克隆起源的并且是离散的 细胞亚群。

The proliferation kinetics of murine and human mononuclear phagocytes will be studied. The relationship between factors which are required for and promote proliferation and those which inhibit proliferation will be defined. It is our belief that once we understand how proliferation is regulated we will be able to alter the host's environment during disease states to mobilize effective numbers of mononuclear phagocytes. No natter how much we try to reduce the burden of pathogenic organisms or tumor burden which these normal host defense cells combat, they will be ineffective if there are too few available. We are also studying mononuclear phagocyte proliferation in order to provide clones of these cells in large enough numbers to study their functional heterogeneity. Using the Flow Cytometer and Cell Sorter, we will study differentiation of mononuclear phagocytes as they proliferate. By removing the growth factors we can also study their differentiation in the absence of proliferation. We plan to study the following markers of differentiation: The rate of adherence of progeny derived from nonadherent bone marrow progenitor cells, the development of phagocytosis and the development of membrane markers for Ia, Fc receptors and C3b receptors. We will be able to determine from these studies whether functions or markers expressed by monouclear phagocytes represent differentiated states through which all cells pass or whether some functions are of clonal origin and unique to a discrete subpopulation of cells.