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MOLECULAR PHENOTYPING BY CYTOMETRY--CYTOKINE EXPRESSION

MOLECULAR PHENOTYPING BY CYTOMETRY--CYTOKINE EXPRESSION
细胞因子表达的分子表型分析
批准号:
2683539
负责人:
CARLETON C STEWART
金额:
$9.84万
依托单位国家:
美国
项目类别:
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-09-23 至 2001-03-31

项目摘要

项目成果

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中文摘要
翻译
描述:拟议研究的目的是开发方法
英文摘要
DESCRIPTION: The purpose of the proposed research is to develop methods to simultaneously immunophenotype and molecular phenotype cells to evaluate cytokine receptor and ligand expression. Hematopoietic cells are derived from a small pool of progenitor cells and differentiate from multipotential stem cells into committed progenitor cells that expand to become functional end cells. Antibodies have been produced to some of the proteins on the membrane of hematopoietic cells but not all of them. Since a unique repertoire of proteins is displayed for each subset along the differentiation pathway, investigators seek to be able to separate and identify specific cell populations using flow cytometry. The investigators hypothesize that hematopoietic cell subsets express the unique repertoire of cytokine receptors whose engagement by ligands regulates both proliferation and differentiation of these cells. To understand the functional aspects of this differentiation process it is proposed to develop the procedures necessary to both immunophenotype and molecular phenotype. Molecular phenotyping is a term applied to describe the process of determining specific nucleic acids sequences inside a cell. Both of these methods are necessary because the specific antibodies to identify certain cell types that are available, yet few are available for measuring cytokine ligands and only some ligand receptors. Molecular phenotyping by in situ PCR combined with immunophenotyping is not yet completely reduced to practice, therefore, some development work is also necessary. The detection of nucleic acid sequences will be performed by fluorescence in situ hybridization in suspension as well as by in situ PCR. The proposal emphasizes the use of flow cytometry for detection, however, microscopic imaging will be used as well. The experiments are designed to provide information that will help understand normal hematopoiesis. The development of a reliable method for molecular phenotyping by flow cytometry will have an enormous impact on basic, translational and clinical research. There are three goals to this study. The first is to develop a reliable method for molecular phenotyping by flow cytometry that can be combined with immunophenotyping. The second is to apply this technology to define the specific cytokine receptor expressed by subsets of hematopoietic progenitor cells. The third goal is to define the ligands produced by subsets of T-cells and monocytes. The methods and results will open a new approach to the interrogation of cellular function.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
Identification and function of host defense cells by flow cytometry.
通过流式细胞术鉴定宿主防御细胞及其功能。
DOI: 10.3109/08820139709048917
发表时间: 1997
期刊: Immunological investigations
影响因子: 2.8
作者: [Stewart,CC]
通讯作者: Stewart,CC
DOI: 10.1002/cyto.990220313
发表时间: 1995-09
期刊: Cytometry
影响因子: --
作者: [E. A. Timm;E. Podniesinski;L. Duckett;J. Cardott;C. Stewart]
通讯作者: E. A. Timm;E. Podniesinski;L. Duckett;J. Cardott;C. Stewart
A Shared Multiparameter Flow Cytometer and Cell Sorter
MOLECULAR PHENOTYPING BY FLOW CYTOMETRYI
  • 批准号:
    3203940
  • 项目类别:
  • 资助金额:
    $6.82万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位:
MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF SOLID TUMORS
  • 批准号:
    3203942
  • 项目类别:
  • 资助金额:
    $7.23万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位:
MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF SOLID TUMORS
  • 批准号:
    2100899
  • 项目类别:
  • 资助金额:
    $8.45万
  • 财政年份:
    1992
  • 负责人:
    CARLETON C STEWART
  • 依托单位:
海外基金