A Shared Multiparameter Flow Cytometer and Cell Sorter

描述(由申请人提供)：流式细胞术实验室(LFC)提供核心研究能力，为RPCI几个级别的研究人员提供服务。该资源旨在开发和测试新的细胞准备程序，开发流式细胞仪的新应用，开发新的流式细胞仪功能，并测试以用户友好和可靠的格式分析复杂多参数数据的新软件。对于研究人员，提供了两种服务。提供了培训和咨询计划，以便个人能够学习执行他们自己的数据采集和分析。这是一个非常成功的项目，因为研究人员对流式细胞术的能力和缺陷有了第一手的了解。有了这种实践经验，研究人员更有能力设计更复杂的实验，并恰当地解释相应的结果。服务部分旨在为需要更高级能力的实验提供专业知识，例如同时进行4-8抗体测定(4-7结合DNA含量测量)或细胞分选。这些任务由流式细胞仪实验室人员执行。由于在过去三年中，在RPCI所使用流式细胞仪的研究人员不断扩大，并且为了满足一些研究人员的要求，这些研究人员希望执行多达八种颜色的荧光测量并增加分选，包括单细胞沉积，因此有必要购买新的先进的流式细胞仪和细胞分选仪。这一新工具将扩大我们目前最大限度利用的工具库存，并提高我们的能力。希望进行六参数以上数据采集的主要用户将接受使用新仪器的培训。这将使那些不需要新文书更先进能力的人能够更有效地利用我们现有的文书并提高其能力。随着用荧光表达载体标记基因的颜色数量增加，现在已达到四种，需要细胞分选的分子生物学家的数量已经成为我们研究所的主要需求。在这一需要中，新的文书将补充我们目前负担过重的文书。近几个月来，该研究所积极开展招聘工作，培养了42名临床医生科学家和药理学和治疗学、癌症遗传学、免疫学、生物物理疗法、结构生物学和预防等学科的系领导。预计在未来一到两年内，科学和临床计划将增加15-20名新的教员，因此，更新流式细胞仪的需求将显著扩大。

DESCRIPTION (provided by applicant): The Laboratory of Flow Cytometry (LFC) provides core research capabilities to serve investigators at RPCI at several levels. The Resource is designed to develop and test new cell preparation procedures, develop new applications of flow cytometry, develop new flow cytometer capabilities and to test new software for analyzing complex multiparameter data in a user friendly and reliable format. For the research investigator, two kinds of services are provided. A training and consultation program is provided so individuals can learn to perform their own data acquisition and analysis. This has been a very successful program because the research investigator gets first hand insight into the capabilities and pitfalls of flow cytometry. With this practical experience, the researcher is better able to design more sophisticated experiments and properly interpret the corresponding results. The service component is designed to provide expertise for experiments that require more advanced capabilities, such as simultaneous 4-8 antibody determinations (4-7 in conjunction with DNA content measurements) or for cell sorting. These tasks are performed by Laboratory of Flow Cytometry personnel. Due to an expansion of research investigators using flow cytometry at RPCI over the last three years and to meet the requirements of some investigators who want to perform up to eight colors of fluorescence measurements and increased sorting including single cell deposition, it is necessary to purchase a new advanced state of the art flow cytometer and cell sorter. This new instrument will expand our current inventory of instruments that are currently maximally utilized as well as increase our capability. Major users who wish to perform more than six-parameter data acquisition will be trained to use the new instrument. This will allow more effective utilization and increase capacity of our current instruments for those who do not require the more advanced capabilities of the new instrument. With the increased number of colors, now up to four, for marking genes with fluorescent expression vectors, the number of molecular biologists that require cell sorting has become a dominant need at our Institute. The new instrument will supplement our current overburdened instrument in this need. In recent months, the Institute mounted aggressive recruitment efforts, resulting in 42 clinician scientists and department leaders in Pharmacology and Therapeutics; Cancer Genetics; Immunology, Biophysical Therapy, Structural Biology and Prevention. It is expected that an additional 15-20 new faculties will be recruited to the scientific and clinical programs within the next year or two, thus, the need for updated flow cytometry will be expanded significantly.