Ligand-receptor-like kinase signalling in Arabidopsis root development

拟南芥根发育中的配体受体样激酶信号传导

• 批准号: BB/H022457/1
• 负责人: Ive De Smet
• 金额: $ 98.85万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH022457%2F1
• 关键词: Ligand; receptor; like; kinase; signalling

Food security represents a major global issue, and it was put forward by the crop scientist Jonathan Lynch that a 'second green revolution' should be a priority. This should focus on root architecture, since root branching critically influences nutrient and water uptake efficiency. However, root architecture has not been a trait selected for by plant breeders in major cereal crops. In addition, in multicellular organisms, cell-cell communication plays a crucial role in patterning, growth and interaction with the environment. For example, a tight control of formative cell divisions - which are mainly asymmetric (stem) cell divisions - to produce tissues and organs and to prevent over-proliferation is crucial for the postembryonic growth and development of plants. This control of formative cell divisions is not only critical in plants, but also in animals to prevent irregular divisions or cancers. We recently identified a protein, the membrane-associated receptor-like kinase ACR4, which is crucial in the control of root architecture and which is a key regulator of formative cell divisions in the root of the model plant Arabidopsis thaliana. ACR4 has originally been described in maize for its role in other processes, and provides an obvious starting point for the detailed analysis of the role of receptor-like kinase signaling pathways in root architecture and in registering and conveying positional information. This research proposal aims to further characterize this ligand/receptor signaling mechanism that can integrate mobile signaling molecules to control formative cell divisions during organogenesis and, as such, provides an excellent tool to study short range, cell-cell communication during growth and development. First, we will identify those transcriptional changes that affect cell identity in the main and lateral root downstream of a ligand-driven cue from neighbouring cells. These data will provide us with a large number of transcriptional markers for other experiments. Second, unequivocal identification of the ligand is crucial to fully understand receptor-like kinase-mediated signaling pathways. One important aspect is how ligand-dependent directional cues from surrounding cells modulate receptor-like kinase signaling. We will identify and localize ligands for the ACR4 receptor-like kinase. Third, the three dimensional structure of eukaryotic membrane proteins has only been determined very limitedly. We will determine the folding and structure of purified receptor domain protein and use that for detailed analyses. Fourth, temporary and reversible phosphorylation of proteins is one of the most important post-translational modifications with a regulatory function, such as the control of transcriptional changes. Therefore, we will identify the substrates of ACR4 during main and lateral root development. Sixth, with the knowledge that we will gain on ligand-receptor-like kinase signaling and what is known about hormone signaling and transcriptional networks in the same processes, we will build a comprehensive model.

粮食安全是一个重大的全球问题，农作物科学家乔纳森·林奇提出，应该优先考虑“第二次绿色革命”。这应该集中在根构型上，因为根的分枝对养分和水分的吸收效率有至关重要的影响。然而，根构型并不是植物育种者在主要粮食作物上选择的性状。此外，在多细胞生物体中，细胞间的通讯在构图、生长和与环境的相互作用中起着至关重要的作用。例如，严格控制形成性细胞分裂--主要是不对称的(干细胞)分裂--以产生组织和器官，并防止过度增殖，对植物胚胎后的生长和发育至关重要。这种对形成细胞分裂的控制不仅在植物中至关重要，在动物中也是如此，以防止不规则分裂或癌症。我们最近发现了一种蛋白，膜相关受体样激酶ACR4，它在控制根构型中起关键作用，是模式植物拟南芥根部形成细胞分裂的关键调节因子。ACR4最初在玉米中被描述为其在其他过程中的作用，并为详细分析受体样激酶信号通路在根结构中的作用以及在登记和传递位置信息中的作用提供了一个明显的起点。这项研究计划旨在进一步表征这种配体/受体信号机制，它可以整合移动信号分子来控制器官发生过程中形成的细胞分裂，从而为研究生长发育过程中的短距离细胞间通讯提供了一个很好的工具。首先，我们将确定在邻近细胞的配体驱动的线索下游的主根和侧根中影响细胞特性的转录变化。这些数据将为我们提供大量的转录标记，用于其他实验。其次，明确的识别配体对于全面理解受体样激酶介导的信号通路至关重要。一个重要的方面是来自周围细胞的配体依赖的定向信号如何调节受体样激酶信号。我们将鉴定和定位ACR4受体样激酶的配体。第三，真核膜蛋白的三维结构的测定非常有限。我们将测定纯化的受体结构域蛋白的折叠和结构，并用于详细分析。第四，蛋白质的暂时和可逆的磷酸化是最重要的翻译后修饰之一，具有调节功能，如控制转录变化。因此，我们将在主根和侧根发育过程中确定ACR4的底物。第六，随着我们对配体受体样激酶信号的了解，以及对激素信号和转录网络在相同过程中的已知，我们将建立一个全面的模型。

项目成果

Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

• DOI: 10.1093/jxb/erv360
• 发表时间: 2015-08
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Czyzewicz N;Shi CL;Vu LD;Van De Cotte B;Hodgman C;Butenko MA;De Smet I
• 通讯作者: De Smet I

Antagonistic peptide technology for functional dissection of CLE peptides revisited.

• DOI: 10.1093/jxb/erv284
• 发表时间: 2015-08
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Czyzewicz N;Wildhagen M;Cattaneo P;Stahl Y;Pinto KG;Aalen RB;Butenko MA;Simon R;Hardtke CS;De Smet I
• 通讯作者: De Smet I

The Arabidopsis thaliana CLAVATA3/EMBRYO-SURROUNDING REGION 26 (CLE26) peptide is able to alter root architecture of Solanum lycopersicum and Brassica napus.

• DOI: 10.1080/15592324.2015.1118598
• 发表时间: 2016
• 期刊: Plant signaling & behavior
• 影响因子: 2.9
• 作者: Czyzewicz N;De Smet I
• 通讯作者: De Smet I

Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants.

• DOI: 10.1007/978-1-4939-6469-7_3
• 发表时间: 2017
• 期刊: Methods in molecular biology
• 作者: Nathan Czyzewicz;Elisabeth Stes;Ive De Smet