MOLECULAR GENETICS OF INFLUENZA VIRUS HEMAGGLUTININ

流感病毒仍然是人类无法控制的病原体之一。 这一建议旨在了解生物合成、结构 和血凝素(HA)糖蛋白的功能，主要是 流感病毒包膜的组成部分。在有关的研究中 直接到病毒的生命周期，分子技术 遗传学将被用来分析HA在体内的生物学作用。 病毒粒子进入宿主细胞和宿主细胞的感染性 宿主机体对感染的免疫反应。对中国传统文化的研究 HA的生物合成将阐明 在加工、成熟和细胞内转运方面 一般来说，真核糖蛋白。这些措施的具体目标是 研究包括： 1.确定透明质酸中有助于 低pH诱导的膜融合活性 分子。 2.分析保守氨基酸在植物生长发育中的作用。 流感病毒粒子萌发过程中HA的胞质尾部。 3.分析蛋白质结构在蛋白质降解中的作用 透明质酸的激活及细胞定位的研究 进行这种切割的蛋白酶。 4.研究流感的细胞免疫反应 通过分析不同的呈现方式来感染病毒 将HA抗原转化为细胞毒淋巴细胞。 5.继续研究蛋白质折叠在蛋白质合成中的作用 透明质酸的胞内转运。 6.识别和表征 HA突变体受阻的胞吐途径。

Influenza virus remains one of the uncontrolled pathogens of man. This proposal is aimed at understanding the biosynthesis, structure and function of the hemagglutinin (HA) glycoprotein, the major component of the influenza virus envelope. In studies pertaining directly to the life cycle of the virus, techniques of molecular genetics will be used to analyze the biological roles of HA in the infectivity and entry of the virion into the host cell and in the host organism's immune response to infection. Studies of the biosynthesis of HA will elucidate the cellular mechanisms that underly the processing, maturation and intracellular transport of eukaryotic glycoproteins in general. The specific aims of these studies are: 1. To identify individual amino acids in HA that contribute to the low pH-induced membrane fusion activity of the molecule. 2. To analyze the role of conserved amino acids in the cytoplasmic tail of HA in the budding of influenza virions. 3. To analyze the role of protein structure in the proteolytic activation of HA and to investigate the location of cellular proteases that carry out this cleavage. 4. To investigate the cellular immune response to influenza virus infection by analyzing different modes of presentation of the HA antigen to cytotoxic lymphocytes. 5. To continue to study the role of protein folding in the intracellular transport of HA. 6. To identify and characterize compartments of the exocytotic pathway in which HA mutants become arrested.