BIOLOGY OF LEISHMANIA IN PHLEBOTOMINE SANDFLIES

白蛉中利什曼原虫的生物学

• 批准号: 3130955
• 负责人: ROBERT B TESH
• 金额: $ 17.32万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130955
• 关键词: Leishmania; disease vectors; enzyme linked immunosorbent assay; host organism interaction; leishmaniasis; life cycle; microorganism genetics; microorganism growth; microorganism immunology; nutrient requirement; nutrition related tag; protozoal antigen; tissue /cell culture

This project is a consortium between scientists at Yale University School of Medicine and Harvard Medical School. The objective of the project is to study the biology of human pathogenic Leishmania in their phlebotomine sandfly vectors. The project will involve the disciplines of parasitology, medical entomology, genetics, biochemistry and immunology. Laboratory reared sandflies will be experimentally infected with a number of different Leishmania types and the following aspects of the parasites' biology in the insects will be studied: nutritive requirements of promastigotes in vivo, their complete life cycle, their effect on the vector, differences in sandfly infectivity among various parasite strains, genetic basis of vector competence in sandflies, identification of stage and species specific antigens present on promastigotes in vivo, development of an ELISA technique for assaying Leishmania infection in sandflies, and investigation of the possibility that genetic recombination can occur among related Leishmania types in the insect vector.

这个项目是由耶鲁大学学院的科学家 医学院和哈佛医学院。 该项目的目标是 研究人类致病性利什曼原虫的生物学特性， 白蛉媒介。 该项目将涉及寄生虫学、 医学昆虫学、遗传学、生物化学和免疫学。 实验室 饲养的白蛉将实验性地感染一些不同的 利什曼原虫类型和以下方面的寄生虫的生物学在 将研究昆虫：体内前鞭毛体的营养需求， 它们的完整生命周期，它们对病媒的影响， 白蛉不同寄生虫株间的感染性，媒介的遗传基础 白蛉的能力，阶段和物种特异性的鉴定 体内前鞭毛体上存在的抗原，ELISA的开发 白蛉利什曼原虫感染检测技术及调查 基因重组可能发生在 昆虫媒介中的利什曼原虫类型。