LIGHT SCATTERING MODELS OF BACTERIA AND VIRUSES 19.

细菌和病毒的光散射模型 19。

• 批准号: 3133998
• 负责人: WILLIAM M MC CLAIN
• 金额: $ 6.61万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-15 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3133998
• 关键词: computer assisted diagnosis; computer simulation; fluorescence polarization; fluorescent dye /probe; light intensity; mathematical model; microorganism classification; model design /development; optical polarization; virus classification

The empirical characterization of bacteria, bacterial spores, viruses, and other macromolecular assemblies by means of their polarized light scattering properties is now far ahead of our understanding of the scattered light patterns upon which the characterization is based. We propose to use recent advances in scattering theory to develop a computer program that will accept as its input a polarizability model of the bacterium, etc., and will give as its output the ten independent orientation averaged polarized scattering patterns that characterize all possible polarization properties of the scattered light. This program will allow one to model the viruses, etc., as they exist in aqueous suspension, free of this preparation artifacts which may exist in electron micrographs. One can also use such models to interpret assembly dynamics that may be followed by light scattering methods. We note that the normal scattering may be simplified and enhanced by the use of preresonant Rayleigh scattering from covalently bound fluorescent tags, and we will prepare for the interpretation of such experiments by appropriate model calculations. This method will be unique in giving accurate orientation and distance information on subunit pairs separated by distances comparable to an optical wavelength. This work may contribute to the automated identification of microorganisms, with applications both in research and in clinical medicine.

细菌、细菌孢子、病毒和 其它大分子集合体通过它们的偏振光 散射特性现在远远领先于我们的理解 表征所基于的散射光图案。 我们 我建议利用散射理论的最新进展来开发一台计算机 程序，将接受作为其输入的极化率模型的 细菌等，并将其输出为十个独立的 取向平均偏振散射图案，其表征所有 散射光的可能偏振特性。 此程序将 允许人们对病毒进行建模，等等，由于它们存在于水悬浮液中， 没有可能存在于电子中的这种制备伪影 显微照片 人们也可以使用这样的模型来解释装配动力学 随后可以进行光散射法。 我们注意到， 散射可以通过使用预共振 瑞利散射从共价结合的荧光标记，我们将 准备用适当的模型解释这些实验 计算。 这种方法将是唯一的，在给予准确的定位 和距离信息的亚基对分开的距离可比 到光波长。 这项工作可能有助于自动化 微生物的鉴定，在研究和 临床医学