LIGHT SCATTERING MODELS OF BACTERIA AND VIRUSES

细菌、细菌孢子、病毒和 其他利用偏振光组装的大分子组件 散射特性现在远远超出了我们对 表征所基于的散射光图案。我们 建议利用散射理论的最新进展来开发一台计算机 程序将接受作为其输入的极化模型 细菌等，并将其作为其输出的十个独立 方向平均偏振散射图案，表征了所有 散射光的可能偏振特性。这一计划将 允许人们模拟病毒等，因为它们存在于水悬浮液中， 没有电子中可能存在的这种制备人工制品 显微照片。人们也可以使用这样的模型来解释装配动力学 紧随其后的是光散射方法。我们注意到，正常的 通过使用预共振剂可以简化和增强散射 共价结合的荧光标记的瑞利散射，我们将 为用合适的模型解释这些实验做好准备 计算。这种方法在准确定位方面将是独一无二的。 以及关于由可比较距离分隔的亚基对的距离信息 到一个光学波长。这项工作可能有助于实现自动化 微生物鉴定及其在研究和生产中的应用 临床医学。

The empirical characterization of bacteria, bacterial spores, viruses, and other macromolecular assemblies by means of their polarized light scattering properties is now far ahead of our understanding of the scattered light patterns upon which the characterization is based. We propose to use recent advances in scattering theory to develop a computer program that will accept as its input a polarizability model of the bacterium, etc., and will give as its output the ten independent orientation averaged polarized scattering patterns that characterize all possible polarization properties of the scattered light. This program will allow one to model the viruses, etc., as they exist in aqueous suspension, free of this preparation artifacts which may exist in electron micrographs. One can also use such models to interpret assembly dynamics that may be followed by light scattering methods. We note that the normal scattering may be simplified and enhanced by the use of preresonant Rayleigh scattering from covalently bound fluorescent tags, and we will prepare for the interpretation of such experiments by appropriate model calculations. This method will be unique in giving accurate orientation and distance information on subunit pairs separated by distances comparable to an optical wavelength. This work may contribute to the automated identification of microorganisms, with applications both in research and in clinical medicine.