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Finding genes that determine variation in sperm morphology and motility

寻找决定精子形态和活力变化的基因

基本信息

批准号：
BB/I02185X/1
负责人：
J Slate
金额：
$ 98.13万
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2012
资助国家：
英国
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FI02185X%2F1
关键词：
Finding genes determine variation sperm

项目摘要

Across different animal taxa, sperm are perhaps the most diverse of all types of cell. However, in all species they have one principal function; to fertilise an egg. In many different biological and medical sub-disciplines there has been considerable interest in how sperm morphology can influence sperm motility (swimming speed) and fertility. We probably all have a 'cartoon' image of sperm with the longest tail swimming faster than sperm with short tails. Therefore, it is perhaps surprising that within most organisms where people have looked at the relationship between sperm length and sperm motility they have either failed to find any relationship, or have only found a relationship with particular components of the sperm (e.g. the head, the midpiece). In our study organism, the zebra finch, which is an important model organism in neurobiology and behavioural ecology, we have shown that there is considerable inter-male variation in sperm traits, but that within males sperm morphology and motility are highly repeatable. We have also demonstrated that males with long sperm have the fastest sperm, and that both sperm length and sperm speed are heritable. Finally (and uniquely) we have shown that there is a genetic correlation between sperm length and sperm motility, which strongly suggests that the relationship is causative rather than because both sperm traits are simultaneously influenced by environmental factors (e.g. diet, condition). While investigating zebra finch sperm biology we have also been at the forefront of the development of zebra finch genomics tools. For example, we constructed the first zebra finch genetic linkage map and we were part of an international team that sequenced and assembled the zebra finch genome. In this project we will integrate the two strands (sperm biology and genomics) of our previous zebra finch work. We will perform genome-wide association studies (GWAS) to map and identify the genes responsible for inter-male differences in sperm morphology and motility. Genes responsible for differences in sperm motility have not yet been identified in any species. We will do this by developing a chip that probes 20,000 genetic markers at once and typing 1000 male birds (of known sperm morphology/motility) with the chip. We will also sequence the transcriptome (which contains the part of the genome that encodes proteins) from the testes of 20 males with short sperm and 20 males with long sperm. These males come from our existing selection lines which already show a large difference in sperm length. By integrating the GWAS and the transcriptomics data we will provide the first ever description of the genes that determine variation in sperm morphology and motility. Therefore, our findings will be relevant to researchers in human fertility medicine, animal breeding/agriculture, conservation biology and evolutionary biology.

在不同的动物类群中，精子可能是所有类型的细胞中最多样化的。然而，在所有物种中，它们都有一个主要功能；使卵子受精在许多不同的生物学和医学分支学科中，人们对精子形态如何影响精子活力（游泳速度）和生育能力有着相当大的兴趣。我们可能都有这样一个“卡通”形象：尾巴最长的精子比尾巴短的精子游得快。因此，也许令人惊讶的是，在大多数生物体中，当人们研究精子长度和精子活力之间的关系时，他们要么没有发现任何关系，要么只发现了与精子的特定组成部分（例如头部，中间部分）的关系。在我们的研究中，斑胸草雀是神经生物学和行为生态学中重要的模式生物，我们已经证明雄性之间的精子特征存在相当大的差异，但雄性精子的形态和运动是高度可重复的。我们还证明，精子长的男性精子速度最快，而且精子长度和精子速度都是可遗传的。最后（也是唯一的），我们已经证明了精子长度和精子活力之间存在遗传相关性，这强烈表明这种关系是因果关系，而不是因为两种精子特征同时受到环境因素（如饮食、条件）的影响。在研究斑胸草雀精子生物学的同时，我们也一直处于斑胸草雀基因组学工具开发的前沿。例如，我们构建了第一个斑胸草雀遗传连锁图谱，我们是一个对斑胸草雀基因组进行测序和组装的国际团队的一部分。在这个项目中，我们将整合我们之前的斑马雀工作的两个方面（精子生物学和基因组学）。我们将进行全基因组关联研究（GWAS），以绘制和鉴定导致男性之间精子形态和活力差异的基因。导致精子活力差异的基因尚未在任何物种中被发现。我们将通过开发一种芯片来实现这一目标，该芯片可以同时探测20,000个遗传标记，并用该芯片对1000只雄性鸟类（已知精子形态/运动）进行分型。我们还将对20名短精子男性和20名长精子男性的睾丸转录组（包含编码蛋白质的基因组部分）进行测序。这些雄性来自我们现有的选择系，这些选择系已经显示出精子长度的巨大差异。通过整合GWAS和转录组学数据，我们将首次描述决定精子形态和活力变异的基因。因此，我们的研究结果对人类生育医学、动物育种/农业、保护生物学和进化生物学的研究人员具有重要的指导意义。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sperm morphology, adenosine triphosphate (ATP) concentration and swimming velocity: unexpected relationships in a passerine bird.

DOI：
10.1098/rspb.2016.1558
发表时间：
2016-08-31
期刊：
Proceedings. Biological sciences
影响因子：
0
作者：
Bennison C;Hemmings N;Brookes L;Slate J;Birkhead T
通讯作者：
Birkhead T

Red Carotenoid Coloration in the Zebra Finch Is Controlled by a Cytochrome P450 Gene Cluster

DOI：
10.1016/j.cub.2016.04.047
发表时间：
2016-06-06
期刊：
CURRENT BIOLOGY
影响因子：
9.2
作者：
Mundy, Nicholas I.;Stapley, Jessica;Slate, Jon
通讯作者：
Slate, Jon

Long sperm fertilize more eggs in a bird.

DOI：
10.1098/rspb.2014.1897
发表时间：
2015-01-22
期刊：
Proceedings. Biological sciences
影响因子：
0
作者：
Bennison C;Hemmings N;Slate J;Birkhead T
通讯作者：
Birkhead T

High-utility conserved avian microsatellite markers enable parentage and population studies across a wide range of species.

DOI：
10.1186/1471-2164-14-176
发表时间：
2013-03-15
期刊：
BMC genomics
影响因子：
4.4
作者：
Dawson DA;Ball AD;Spurgin LG;Martín-Gálvez D;Stewart IR;Horsburgh GJ;Potter J;Molina-Morales M;Bicknell AW;Preston SA;Ekblom R;Slate J;Burke T
通讯作者：
Burke T