PITUITARY LACTOGENIC AND GONADOTROPIC HORMONES

垂体催乳激素和促性腺激素

• 批准号: 3150720
• 负责人: CHOH H LI
• 金额: $ 11.11万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3150720
• 关键词: carbohydrates; chemical association; chemical structure function; chromatography; circular dichroism; conformation; disulfide bond; follicle stimulating hormone; hormone analog; human tissue; immunochemistry; luteinizing hormone; plasmin; prolactin; proteolysis; spectrometry; thermolysin; trypsin; tryptophan

The long term goal of this project is to advance our general understanding of the structure-function relationships in pituitary prolactins and gonadotropins. This proposal outlines specific groups of experiments, designed to broaden our data base with respect to both conserved and non-conserved primary, secondary, tertiary and quaternary structural features, and further correlation of these features with the presence of biochemical parameters. The recent discovery and preliminary descriptions of at least 3 atypical forms of prolactin will be exploited to study the structural and functional roles of the various disulfide bonds and tryptophan residues in this class of hormones. The complementation of prolactin fragments will be further investigated in order to prepare semi-synthetic analogues. An extensive conformational study of equine-LH is of particular interest in view of this molecule's intrinsic FSH-like activity. These experiments will include the novel application of second-order spectroscopy. Of equal importance is a thorough conformational evaluation of partially deglycosylated ovine-LH, its subunits, and hybrids formed with native oLH subunits.

这个项目的长期目标是增进我们对 脑下垂体催乳素的结构与功能关系 促性腺激素。这份提案概述了几组具体的实验， 旨在扩大我们的数据库，包括保守的和 非保守的一级、二级、三级和四级结构 特征，以及这些特征与存在的 生化指标。最新发现及初步描述 将利用至少3种非典型催乳素形式来研究 不同二硫键的结构和功能作用 色氨酸是这类激素中的残留物。的互补性 将对催乳素片段进行进一步研究，以准备 半合成类似物。马-黄体生成素的广泛构象研究 特别令人感兴趣的是这种分子固有的类FSH 活动。这些实验将包括新的应用 二阶光谱学。同样重要的是彻底的 部分脱糖的绵羊黄体生成素的构象评价 亚基，以及与天然olh亚基形成的杂交种。