ACTH AND RELATED PEPTIDES

ACTH 及相关肽

• 批准号: 3267845
• 负责人: CHOH H LI
• 金额: $ 11.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-03-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3267845
• 关键词: adrenocorticotropic hormone; autoradiography; chemical structure function; chromatography; high performance liquid chromatography; hormone analog; hormone inhibitor; lipolysis; melanocyte stimulating hormone; peptide hormone biosynthesis; radioimmunoassay; radiotracer; reversed phase chromatography; scintillation counter; steroid biosynthesis; tritium

Our objective is to study the structure-activity relationship of corticotropins (ACTHs), lipotropins (LPHs), endorphins (EPs), melanotropins (MSHs) and related peptides. It is planned to isolate ACTH, Beta-LPH, Beta-EP and related peptides from elephant pituitary glands and to determine their amino acid sequences. Once the structure is known elephant ACTH, Beta-EP and related peptides will be synthesized by the solid-phase method. It is also planned to synthesize dogfish and amphibian ACTHs. When the synthetic peptides become available, their biological properties are investigated. Alphah-ACTH-(7-38) [corticotropin-inhibiting peptide (CIP)], the naturally occurring inhibitor to ACTH, is a weak antagonist. It is hope to synthesize analogs of CIP with higher inhibitory activity. Similarly, it is planned to synthesize analogs of Betah-EP-(1-27), [Beta-EP inhibiting peptide (BetaEIP)], the naturally occurring inhibitor to Beta-EP-induced analgesia, with higher inhibitory activity. For the development of sensitive and specific radioimmunoassays (RIAs) for rat and human Gamma-LPH, it is necessary to synthesize these two Gamma-LPHs by the solid-phase method. ACTH and related peptides are known to exhibit no three dimentional structures in water. It is planned to investigate the presence of a tertiary structure in aqueous solutions of human ACTH, Beta-MSH and Alpha-MSH by difference absorption spectroscopy of enzymic digests of the hormones.

我们的目标是研究结构-活性关系 促肾上腺皮质激素 (ACTH)、促脂激素 (LPH)、内啡肽 (EP)、促黑激素 （MSH）和相关肽。 计划分离ACTH、Beta-LPH、 来自大象脑垂体的 Beta-EP 和相关肽 确定它们的氨基酸序列。 一旦了解了大象的结构 固相合成ACTH、Beta-EP及相关肽 方法。 还计划合成角鲨和两栖动物 ACTH。 当合成肽可用时，它们的生物学特性 被调查。 Alphah-ACTH-(7-38) [促肾上腺皮质激素抑制肽 (CIP)]，天然 ACTH 的抑制剂，是一种弱拮抗剂。 希望能够 合成具有更高抑制活性的CIP类似物。 同样，它 计划合成Betah-EP-(1-27)的类似物，[Beta-EP抑制 肽（BetaEIP）]，天然存在的 Beta-EP 诱导抑制剂 镇痛，具有较高的抑制活性。 开发灵敏且特异的放射免疫测定法 (RIA) 大鼠和人的Gamma-LPH，需要合成这两种Gamma-LPH 通过固相法。 已知 ACTH 和相关肽不表现出三维 水中的结构。 计划调查是否存在 人 ACTH、Beta-MSH 和的水溶液中的三级结构 α-MSH 通过酶消化物的差异吸收光谱 荷尔蒙。