ACTH AND RELATED PEPTIDES

我们的目标是研究其构效关系。 促肾上腺皮质激素(ACTHs)、促脂激素(LPHs)、内啡肽(EPs)、促黑素激素 (MSHS)和相关多肽。计划分离ACTH、Beta-LPH、 大象脑下垂体和TO的β-EP和相关多肽 确定它们的氨基酸序列。一旦结构为人所知，大象 ACTH、β-EP及相关多肽的固相合成 方法。它还计划合成角鱼和两栖ACTH。 当合成肽可用时，它们的生物学特性 都进行了调查。 α-ACTH-(7-38)[促肾上腺皮质激素抑制肽(CIP)] 对ACTH有抑制作用，是一种弱拮抗剂。我们有希望 合成具有较高抑制活性的CIP类似物。同样，它 计划合成BETAH-EP-(1-27)的类似物，[Beta-EP抑制 多肽(BetaEIP)--β-EP诱导的天然抑制物 止痛作用，具有较高的抑制活性。 用于开发灵敏和特异的放射免疫分析(RIA) 大鼠和人的γ-LPH，合成这两种γ-LPH是必要的 采用固相法。 已知促肾上腺皮质激素和相关多肽没有三维结构。 水中的建筑物。计划调查一名 人ACTH、β-MSH和ACTH水溶液中的三级结构 α-MSH差示吸收光谱分析 荷尔蒙。

Our objective is to study the structure-activity relationship of corticotropins (ACTHs), lipotropins (LPHs), endorphins (EPs), melanotropins (MSHs) and related peptides. It is planned to isolate ACTH, Beta-LPH, Beta-EP and related peptides from elephant pituitary glands and to determine their amino acid sequences. Once the structure is known elephant ACTH, Beta-EP and related peptides will be synthesized by the solid-phase method. It is also planned to synthesize dogfish and amphibian ACTHs. When the synthetic peptides become available, their biological properties are investigated. Alphah-ACTH-(7-38) [corticotropin-inhibiting peptide (CIP)], the naturally occurring inhibitor to ACTH, is a weak antagonist. It is hope to synthesize analogs of CIP with higher inhibitory activity. Similarly, it is planned to synthesize analogs of Betah-EP-(1-27), [Beta-EP inhibiting peptide (BetaEIP)], the naturally occurring inhibitor to Beta-EP-induced analgesia, with higher inhibitory activity. For the development of sensitive and specific radioimmunoassays (RIAs) for rat and human Gamma-LPH, it is necessary to synthesize these two Gamma-LPHs by the solid-phase method. ACTH and related peptides are known to exhibit no three dimentional structures in water. It is planned to investigate the presence of a tertiary structure in aqueous solutions of human ACTH, Beta-MSH and Alpha-MSH by difference absorption spectroscopy of enzymic digests of the hormones.