PITUITARY LACTOGENIC AND GONADOTROPIC HORMONES

垂体催乳激素和促性腺激素

• 批准号: 3224467
• 负责人: CHOH H LI
• 金额: $ 10.52万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3224467
• 关键词: carbohydrates; chemical association; chemical structure function; chromatography; circular dichroism; conformation; disulfide bond; follicle stimulating hormone; hormone analog; human tissue; immunochemistry; luteinizing hormone; plasmin; prolactin; proteolysis; spectrometry; thermolysin; trypsin; tryptophan

The long term goal of this project is to advance our general understanding of the structure-function relationships in pituitary prolactins and gonadotropins. This proposal outlines specific groups of experiments, designed to broaden our data base with respect to both conserved and non-conserved primary, secondary, tertiary and quaternary structural features, and further correlation of these features with the presence of biochemical parameters. The recent discovery and preliminary descriptions of at least 3 atypical forms of prolactin will be exploited to study the structural and functional roles of the various disulfide bonds and tryptophan residues in this class of hormones. The complementation of prolactin fragments will be further investigated in order to prepare semi-synthetic analogues. An extensive conformational study of equine-LH is of particular interest in view of this molecule's intrinsic FSH-like activity. These experiments will include the novel application of second-order spectroscopy. Of equal importance is a thorough conformational evaluation of partially deglycosylated ovine-LH, its subunits, and hybrids formed with native oLH subunits.

这个项目的长期目标是促进我们的总体认识 垂体催乳素的结构-功能关系， 促性腺激素 该提案概述了具体的实验组， 旨在扩大我们的数据库， 非保守的一级、二级、三级和四级结构 特征，以及这些特征与 生化参数 最近的发现和初步描述 将利用至少3种非典型形式的催乳素来研究 各种二硫键的结构和功能作用， 这类激素中的色氨酸残基。 的互补 将进一步研究催乳素片段，以制备 半合成类似物。 马LH构象的广泛研究 由于该分子的内在FSH样 活动 这些实验将包括新的应用， 二阶光谱 同样重要的是， 部分去糖基化绵羊LH的构象评价， 亚基和与天然oLH亚基形成的杂交体。