HCS (HPL), HGH AND RELATED PROTEINS

HCS (HPL)、HGH 和相关蛋白质

• 批准号: 3226111
• 负责人: CHOH H LI
• 金额: $ 11.8万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3226111
• 关键词: chemical structure function; conformation; drug design /synthesis /production; gene complementation; human tissue; immunochemistry; peptide hormone; prolactin; radioimmunoassay; somatomammotropin; somatotropin; thermostability

The long-term goal of this project is to define the structure-function relationships of pituitary growth hormone, HCS(hPL) and related proteins. The current proposal seeks to exploit the physico-chemical differences between hGH and HCS (hPL) with the intent of sorting out those aspects which specifically prevent HCS (hPL) from being a potent growth promoting agent. New optical techniques will be used to search the native molecules for additional differences. Non-covalent recombinants (complementation reactions) will be employed to establish precisely which residues between sequence positions 135 and 191 are responsible for interacting with the first 134 residues to create the Alpha-helix content and micro-environments of Trp86 and Tyr143, characteristic of each molecule. Conformational similarities between hGH and HCS (hPL) will be correlated with known prolactin conformations in order to establish which are responsible for the nearly equivalent lactogenic potencies of these molecules. Similarly, elephant growth hormone will be isolated and its conformation studied to further identify secondary and tertiary structural characteristics which are highly conserved by all potent growth hormones. All fragments generated during these studies, whether produced from natural products, or synthesized, will be screened for the ability to inhibit any of the known biochemical functions of hGH.

该项目的长期目标是定义结构功能 垂体生长激素、HCS(hPL) 和相关蛋白的关系。 目前的提案旨在利用物理化学差异 hGH 和 HCS (hPL) 之间的研究，旨在理清这些方面 它专门阻止 HCS (hPL) 成为有效的生长促进剂 代理人。 新的光学技术将用于搜索天然分子 以获得额外的差异。 非共价重组体（互补 反应）将被用来精确确定之间的残基 序列位置 135 和 191 负责与 前 134 个残基用于创建 α 螺旋内容和微环境 Trp86 和 Tyr143，每个分子的特征。 构象的 hGH 和 HCS (hPL) 之间的相似性将与已知的 催乳素构象以确定负责 这些分子的催乳效力几乎相同。 相似地， 将分离大象生长激素并研究其构象 进一步确定二级和三级结构特征 所有有效的生长激素都高度保守。 所有碎片 在这些研究过程中产生的，无论是从天然产品中产生的，还是 合成的，将筛选抑制任何已知的能力 hGH 的生化功能。