PSYCHOPHARMACOLOGICALLY SIGNIFICANT PEPTIDES

具有精神药理学意义的肽

• 批准号: 3207918
• 负责人: CHOH H LI
• 金额: $ 10.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1988-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3207918
• 关键词: brain; cell membrane; chemical structure function; cyclazocine; drug addiction antagonist; drug adverse effect; drug design /synthesis /production; drug metabolism; glioma; human tissue; hybrid cells; morphine; neoplastic cell culture for noncancer research; neuroblastoma; opiate alkaloid; peptides; physical separation; pituitary gland; psychopharmacologic agent; radioimmunoassay; synthetic peptide

A general goal of this proposed project is to investigate the function of beta-endorphin (Beta-EP) and related psychopharmacologically significant peptides at the molecular level. The specific aims are as follows: (a) To investigate on the isolation and characterization of the opioid and non-opioid beta-EP binding proteins (receptors) from membrane preparations of rat brains, mouse neuroblastoma x glioma hybrid cells (NG108-15 and N18TG2), human glioblastoma SF126 cells and human neuroblastoma IMR-32 cells using a photoaffinity reagent 2-nitro-4-azidophenylsulfenyl chloride specific to label tryptophan residue; (b) To synthesize two analogs ([Trp-4]-beta-h-EP; [D-Trp-4]-beta-h-EP) by solid-phase method to use for photolabel probes of the opioid domains in the beta-h-EP molecule. Synthetic [Trp-27]-beta-h-EP will be used for photolabel probes of non-opioid binding sites; (c) To further characterize biologically the naturally occurring beta-EP-inhibiting peptide [beta-EIP, beta-EP-(1-27)] and the synthetic analogs with inhibiting beta-EP analgesia property by radioreceptor binding assay using rat brain membrane preparations and tritiated dihydromorphine, [D-Ala-2, D-Leu-5]-enkephalin, ethylketocyclazocine, beta-EP and diprenorphine as primary ligands as well as by bioassays using guinea pig ileum, rat, mouse and rabbit vas deferens preparations; (d) to test inhibiting activities of beta-h-EP-(1-27) in other biological systems such as prolactin-releasing activity of beta-EP; and (e) to isolate, synthesize and characterize beta-EP from elephant pituitary glands.

这个提议的项目的总体目标是调查的功能

项目成果

Intravenous beta-endorphin: behavioral and physiological effects in conscious monkeys.

静脉内β-内啡肽：对有意识的猴子的行为和生理影响。

• DOI: 10.1016/0196-9781(89)90104-6
• 发表时间: 1989
• 期刊: Peptides
• 影响因子: 3
• 作者: Cuthbert,BN;Holaday,JW;Meyerhoff,J;Li,CH
• 通讯作者: Li,CH

Preparation and properties of tritiated human [Tyr18, Trp27]-beta-endorphin.

氚化人[Tyr18，Trp27]-β-内啡肽的制备和性质。

• 发表时间: 1985
• 期刊: International journal of peptide and protein research
• 作者: HammondsJr,RG;Li,CH
• 通讯作者: Li,CH

Studies on opioid receptor selectivity of beta-endorphin antagonists.

β-内啡肽拮抗剂阿片受体选择性的研究。

• DOI: 10.1111/j.1399-3011.1985.tb03188.x
• 发表时间: 1985
• 期刊: International journal of peptide and protein research
• 作者: HammondsJr,RG;Li,CH
• 通讯作者: Li,CH

Beta-endorphin. Biological activity of synthetic analogs with analgesia inhibiting property in rat vas deferens and guinea pig ileum assays.

β-内啡肽。

• DOI: 10.1111/j.1399-3011.1985.tb02178.x
• 发表时间: 1985
• 期刊: International journal of peptide and protein research
• 作者: Ho,CL;Li,CH
• 通讯作者: Li,CH

Effect of centrally and peripherally administered beta-endorphin on food intake in rats.

中枢和外周给药β-内啡肽对大鼠食物摄入的影响。

• DOI: 10.1111/j.1399-3011.1989.tb00702.x
• 发表时间: 1989
• 期刊: International journal of peptide and protein research
• 作者: Triscari,J;Nelson,D;Vincent,GP;Li,CH
• 通讯作者: Li,CH