ACTH AND RELATED PEPTIDES

是次续期申请的主要目的是研究 促肾上腺皮质激素、促黑激素、β-内啡肽的构效关系 β-促脂素及相关多肽。具体目标如下： (1)分离β-促脂激素(β-LPH)、促肾上腺皮质激素(ACTH)和β-内啡肽， (β-EP)从马和大象的脑垂体腺中提取，并测定它们的 氨基酸序列。(2)合成马、象、鸵鸟ACTH 和β-内啡肽的固相法。要确定主要的 鸵鸟脑下垂体β-LPH的结构为了研究 马、象的脂解、类固醇生成和免疫活性 和鸵鸟Beta-LPH，并比较它们的生物活性 其他物种。(3)测定银杏叶提取物的促醛固酮活性 ACTH、α-MSH、β-MSH和β-LPH的各种合成类似物。至 固相法合成马β-MSH和[DES-Asp1]-Beta-MSH 并对合成产物的生物活性进行研究。 具有促黑、降脂和促类固醇的功效。要继续 ACTH和MSH多肽的合成：a)至 进一步定义荷尔蒙的结构特征是必不可少的 用于生物功能；b)制备具有选择性的多肽 抑制ACTH或MSH的生物活性；c)制备ACTH类似物 主要具有皮质酮或醛固酮刺激活性。至 合成不同类型的人AS-LPH并研究其生物活性。至 建立人体AS-LPH放射免疫分析方法。(四)净化和 膜上β-EP结合蛋白或受体的鉴定 从大鼠或/和牛的匀浆以及从 神经母细胞瘤X胶质瘤杂交细胞。

The chief objective of this renewal application is to study the structure-activity relationship of ACTH, melanotropins, beta-endorphin, Beta-lipotropin and related peptides. The specific aims are as follows: (1) To isolate Beta-lipotropin, (Beta-LPH), ACTH and beta-endorphin, (Beta-EP) from horse and elephat pituitary glands and to determine their amino acid sequences. (2) To synthesize horse, elephant and ostrich ACTH and Beta-endorphin by the solid-phase method. To determine the primary structure of Beta-LPH from ostrich pituitary glands. To study the lipolytic, steroid-ogenic and immunological activities of horse, elephant and ostrich Beta-LPH and compare their biological activities with that from other species. (3) To determine the aldosterone-stimulating activity of various synthetic analogs of ACTH, Alpha-MSH, Beta-MSH and Beta-LPH. To synthesize horse Beta-MSH and [Des-Asp1]-Beta-MSH by the solid phase method and to study the biological activity of the synthetic products for their melanotropic, lipolytic and steroidogenic potency. To continue the synthesis of ACTH and MSH peptides with the following objectives: a) to further define those structure features of the hormone which are essential for the biological functions; b) to prepare peptides which selectively inhibit biological activity of ACTH or MSH and c) to prepare ACTH analogs with mainly corticosterone - or aldosterone-stimulating activity. To synthesize human varies as-LPH and study its biological activity. To develop radioimmunoassay for human varies as-LPH. (4) To purify and characterize the binding protein or the receptor for Beta-EP in membrane preparation from rat or/and bovine homogenates as well as from neuroblastoma X glioma hybrid cells.