MURINE LYME BORRELIOSIS

莱姆病是一种最近描述的螺旋体疾病， 在美国和欧洲的许多地区都很流行。 很少 对易感性、潜伏期、免疫学 病理学和发病机制。 我们对这些问题的无知导致了 最佳诊断和治疗的不确定性。 可靠的动物模型 是必要的。 该建议的目标是通过以下方法研究感染的小鼠模型： 莱姆病的病原体伯氏疏螺旋体及其使用 获得管理信息的模式，两个主要决定因素， 感染是B类。burgdorferi菌株和宿主的遗传背景。 在 本实验研究了B对敏感品系小鼠的感染过程。 将通过研究剂量的影响来研究burgdorferi 微生物、注射途径和培养处死日期 积极和存在的细菌和炎症的器官。 蜂窝 并评估感染小鼠的体液免疫应答。 然后，在模型的初始表征中获得的信息可以 可以用来探讨两个重要问题：基因决定的 各种小鼠品系的易感性和 不同种类的微生物。 从这项工作中获得的信息将提高我们对 莱姆病通过获得信息的顺序事件后， 最初的感染，器官感染，变化的螺旋体，因为它 残留在体内，并在感染中诱发病理变化。 这些 调查也将有助于我们理解B. burgdorferi能够建立潜伏期和逃避，或者至少达成协议 宿主的免疫反应

Lyme disease is a recently described spirochetal illness that is increasing in prevalence in many areas of the United States and Europe. Very little is known about basic issues such as susceptibility, latency, immunology, pathology, and pathogenesis. Our ignorance about these questions leads to uncertainty about optimal diagnosis and treatment. A reliable animal model is needed. The goal os this proposal is to investigate a mouse model of infection by Borrelia burgdorferi, the causative organism of Lyme disease, and to use the model to gain information on management, two major determinants of infection are B. burgdorferi strain and genetic background of the host. In this project the course of infection of a susceptible mouse strain by B. burgdorferi will be studied by investigating the influence of dose of organism, the route of injection, and day of sacrifice on culture positively and presence of bacteria and inflammation in organs. Cellular and humoral immune responses in infected mice will be assessed. Information gained in this initial characterization of the model can then be used to explore two important issues: genetically determined susceptibility in various mouse strains and variability in infectivity in different strains of the organism. The information gained from this work will improve our understanding of Lyme borreliosis by gaining information on the sequence of events after initial infection, the organs infected, changes in the spirochete as it remains in vivo, and pathological changes induced in the infection. These investigations will also aid in our understanding of the means by which B. burgdorferi is able to establish latency and evade, or at least make terms with, the host immune response.