PATHOGENESIS OF NEUROBORRELIOSIS

神经疏螺旋体病的发病机制

• 批准号: 2892026
• 负责人: ANDREW R PACHNER
• 金额: $ 21.34万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2892026
• 关键词: Borrelia; Macaca mulatta; borreliosis; host organism interaction; immunocytochemistry; infectious meningitis; microorganism immunology; neuropathology; pathologic process; polymerase chain reaction; virulence

Lyme neuroborreliosis (LNB) is an increasingly prevalent cause of neurological disease in the world. The infection results in chronic meningitis, cranial nerve lesions, chronic disorders of memory and cognitive functioning, and peripheral nerve damage. Very little is known about pathogenesis of the infection in the central nervous system (CNS). This ignorance about the pathogenesis of LNB has prompted laborious and excessively expensive diagnostic procedures, as well as bizarre and frequently toxic treatments: For instance, we do not know: how frequently the spirochete invades the CNS after intradermal injection how long the infection persists in the CNS the CNS cell type and location infected whether the immune system plays a significant role in the CNS infection whether spirochetes invade peripheral nerves This project will address these areas of ignorance, using a model of LNB in Rhesus macaques recently developed in our laboratory. The information gained will lead to an understanding of pathogenesis as well as the development of rationale diagnostic strategies and safe, effective therapy. It will also lead to a greater understanding of chronic infections of the CNS.

莱姆病神经疏螺旋体病（LNB）是一种日益普遍的原因， 最严重的神经系统疾病 感染导致慢性 脑膜炎、脑神经损伤、慢性记忆障碍和 认知功能和周围神经损伤 之甚少 已知中枢神经系统感染的发病机制 系统（CNS）。 这种对LNB发病机制的无知， 促使费力和过于昂贵的诊断程序， 以及奇怪的、经常有毒的治疗方法： 例如，我们不知道： 皮内注射后螺旋体侵入中枢神经系统的频率 注射 感染在中枢神经系统中持续多久 CNS细胞类型和感染部位 免疫系统是否在中枢神经系统中起重要作用 感染 螺旋体是否侵入周围神经 这个项目将解决这些领域的无知，使用一个模型， LNB在恒河猴最近在我们的实验室发展。 的 获得的信息将导致对发病机制的理解， 以及制定合理的诊断策略和安全， 有效的治疗。 它也将导致更好地理解 CNS的慢性感染。

项目成果

Central and peripheral nervous system infection, immunity, and inflammation in the NHP model of Lyme borreliosis.

莱姆疏螺旋体病 NHP 模型中的中枢和周围神经系统感染、免疫和炎症。

• 发表时间: 2001
• 期刊: Annals of neurology
• 影响因子: 11.2
• 作者: Pachner,AR;Cadavid,D;Shu,G;Dail,D;Pachner,S;Hodzic,E;Barthold,SW
• 通讯作者: Barthold,SW

Isolation of DNA after extraction of RNA To detect the presence of Borrelia burgdorferi and expression of host cellular genes from the same tissue sample.

提取 RNA 后分离 DNA 检测同一组织样本中伯氏疏螺旋体的存在以及宿主细胞基因的表达。

• DOI: 10.1128/jcm.37.6.2087-2089.1999
• 发表时间: 1999
• 期刊: Journal of clinical microbiology
• 影响因子: 9.4
• 作者: Amemiya,K;Schaefer,H;Pachner,AR
• 通讯作者: Pachner,AR

Humoral immune response associated with lyme borreliosis in nonhuman primates: analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins.

与非人灵长类莱姆疏螺旋体病相关的体液免疫反应：通过免疫印迹和超声或重组蛋白的酶联免疫吸附测定进行分析。

• DOI: 10.1128/cdli.9.6.1348-1355.2002
• 发表时间: 2002
• 期刊: Clinical and diagnostic laboratory immunology
• 作者: Pachner,AR;Dail,D;Li,L;Gurey,L;Feng,S;Hodzic,E;Barthold,S
• 通讯作者: Barthold,S