MALIGNANT BEHAVIOR AND CELLULAR ANTIGEN EXPRESSION

细胞遗传学研究： (1)发现一种新的MPC相关易位类型。滑雪道 携带12号染色体的片段发生了偏着丝粒倒位。这个 倒置片段被移位到15号染色体上。 15号染色体是C-myc定位的D带。 (2)通过对c-myc和IgH进行分类，确定了c-myc和IgH的定位 MPC相关的t(12；15)易位染色体及其DNA杂交 并有相关的调查。C-myc的取向为着丝粒5‘-3’-端粒 IgH为着丝粒-CH-VH-端粒。 (3)inv.Rb(6；15)染色体已证实 携带kappa基因的6号染色体片段被转位到 MPC相关变异易位中的15号染色体并列 以C-myc为尾巴到头部的时尚。 (4)t(15；6)染色体上基因的顺序为着丝粒-5‘-c-myc-3’ PVT-1-CK-端粒。 (5)6号染色体上的Ig-kappa定位也已确定： 着丝粒VK-CK-端粒。 分子研究： (1)ABPC 45缺失MPC的分子分析显示 C-myc基因参与了多种易位/重组事件 这将癌基因置于IgH链基因增强子的控制之下。 (2)在大鼠中，c-myc基因已被分配到7号染色体上。 将免疫球蛋白重链和kappa轻链基因定位到染色体上 分别为6和4。6号和7号染色体参与了相互作用 自发性大鼠的染色体易位特征 免疫细胞瘤。我们已经证明了染色体上的断裂点 7号染色体位于c-myc癌基因编码区之外。 (3)我们发现，将前病毒DNA插入附近 C-myc和其他15号染色体(c-sis、int-1、mlvi-1、mlvi-2)或 17号染色体(Pim-1)相关癌基因是一种罕见的癌基因。 McF和MoMuLV病毒诱导小鼠T细胞淋巴瘤/白血病和IS 与15-三体无关。 (4)我们已经证明在人浆细胞白血病中c-myc 癌基因扩增25到30倍，其中20倍增长 转录活性。(M)

Cytogenetic Studies: (1)\A new type of MPC-associated translocation was identified. The IgH carrying chromosome 12 segment underwent a paracentric inversion. The inverted segment was translocated onto chromosome 15. The breakpoint on chromosome 15 is band D where the c-myc was mapped. (2)\The orientation of c-myc and IgH was determined by sorting the MPC-associated t(12;15) translocation chromosome and hybridizing its DNA with relevant probes. The orientation c-myc is centromere 5' -\3'-telomere and that of IgH is centromere-CH-VH-telomere. (3)\In situ hybridization on inv. Rb(6;15) chromosome has established that the chromosome 6 segment that carries the kappa gene is translocated onto chromosome 15 in the MPC-associated variant translocation and is juxtaposed to c-myc in a tail to head fashion. (4)\The order of genes on the t(15;6) chromosome is centromere-5'-c-myc-3' pvt-1-CK-telomere. (5)\The orientation Ig-kappa on chromosome 6 was also established: centromere VK-CK-telomere. Molecular Studies: (1)\The molecular analysis of the ABPC 45 deletion MPC revealed that the c-myc gene has been involved in multiple translocation/recombination events which placed the oncogene under the control of the IgH chain gene enhancer. (2)\In the rat, the c-myc gene has been assigned to chromosome 7. We have mapped the immunoglobulin heavy and kappa light chain genes to chromosomes 6 and 4, respectively. Chromosomes 6 and 7 take part in the reciprocal chromosomal translocation characteristic to the spontaneous rat immunocytomas. We have demonstrated that the chromosomal breakpoints on the chromosome 7 fall outside the coding region of the c-myc oncogene. (3)\We have found that the insertion of the proviral DNA into the vicinity of c-myc and other chromosome 15 (c-sis, int-1, Mlvi-1, Mlvi-2) or chromosome 17 (pim-1) associated oncogenes is an infrequent event in the MCF and MoMuLV virus induced murine T-cell lymphoma/leukemia and is independent of 15-trisomy. (4)\We have demonstrated that in human plasma cell leukemia the c-myc oncogene is amplified 25 to 30 fold, with a 20-fold increase in transcriptional activity. (M)