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13TSB_SynBio- Novel Bacterial Hosts for Biobutanol Production

13TSB_SynBio-用于生物丁醇生产的新型细菌宿主

基本信息

批准号：
BB/L004356/1
负责人：
Nigel Minton
金额：
$ 31.37万
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2013
资助国家：
英国
起止时间：
2013 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FL004356%2F1
关键词：
13TSB_SynBio Novel Bacterial Hosts Biobutanol

项目摘要

Energy insecurity, global warming and fluctuations in oil prices and has resulted a rapid increase in the demand for the biofuel, bioethanol. However, the alcohol biobutanol is widely acknowledged as a 'superior biofuel'. It is traditionally produced via a sugar-based fermentation process using a bacterium Clostridium acetobutylicum. The process, however, remains uneconomic due to feedstock prices, which represents 60-80% of production costs.Another member of the same family of bacteria, Clostridium pasteurianum is a robust, fast growing bacterium which is also able to grow on glycerol as well as sugars and produce valuable 3-carbon (1,3 propanediol) and 4-carbon chemicals (butyrate & butanol). The microbe has tremendous potential as a fermentation host but has not been exploited due to its limited substrate range and mixed array of chemical products that result in low butanol yield compared to current commercial. In this project we aim to use synthetic biology to introduce new metabolic pathways for starch utilisation and butanol production into C. pasteurianum with the aim to produce butanol in high yield from starch. Genetic manipulation based on gene knockout will also be used to inactivate competing pathways. The partners have extensive experience with solventogenic Clostridia. Nottingham's CRG has developed an impressive range of gene tools and recently completed the determination of the complete genetic blueprint (genome sequence) of C. pasteuranium. GBL has identified novel pathways for both starch degradation and butanol production from its unique collection of commercial strains and leads recommercialisation efforts for the butanol fermentation. This collaboration between GBL and GRG enables, for the first time, a targeted and rational approach for strain improvement in a novel host using synthetic biology and metabolic engineering. This project clearly demonstrates the potential application of synthetic biology for industrial biotechnology. Fermentation performance from the engineered C. pasteurianum strains will be benchmarked against GBL's best performing commercial strains and if promising taken forward for further development. The project output will also support additional projects with this microbe focused on extending the substrate range to include cellulosic feedstocks and developing glycerol metabolism for the production of 3-carbon chemicals.

能源不安全、全球变暖和石油价格的波动导致对生物燃料、生物乙醇的需求迅速增加。然而，酒精生物丁醇被广泛认为是一种“优越的生物燃料”。传统上，它是通过使用醋酸丁酸梭菌的糖基发酵过程生产的。然而，由于原料价格的原因，该工艺仍然不经济，原料价格占生产成本的60-80%。巴氏梭菌是同一细菌家族的另一个成员，它是一种强壮、生长迅速的细菌，它也能在甘油和糖上生长，并产生有价值的3碳（1,3丙二醇）和4碳化学物质（丁酸盐和丁醇）。这种微生物作为发酵宿主具有巨大的潜力，但由于其底物范围有限，化学产品的混合阵列导致丁醇产量低于目前的商业生产，因此尚未得到开发。在这个项目中，我们的目标是利用合成生物学为巴氏杆菌引入淀粉利用和丁醇生产的新代谢途径，目的是从淀粉中高产地生产丁醇。基于基因敲除的基因操作也将用于灭活竞争途径。合作伙伴在溶剂性梭菌方面有丰富的经验。诺丁汉大学的基因研究小组开发了一系列令人印象深刻的基因工具，最近完成了巴氏杆菌完整基因蓝图（基因组序列）的测定。GBL已经从其独特的商业菌株收集中确定了淀粉降解和丁醇生产的新途径，并领导了丁醇发酵的再商业化努力。GBL和GRG之间的合作首次利用合成生物学和代谢工程为新型宿主的菌株改良提供了一种有针对性和合理的方法。这个项目清楚地展示了合成生物学在工业生物技术方面的潜在应用。工程巴氏梭菌菌株的发酵性能将与GBL表现最佳的商业菌株进行基准测试，如果有希望进一步开发。该项目的产出还将支持该微生物的其他项目，重点是扩大底物范围，包括纤维素原料和开发用于生产3碳化学品的甘油代谢。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Complete Genome Sequence of the Nitrogen-Fixing and Solvent-Producing Clostridium pasteurianum DSM 525.

DOI：
10.1128/genomea.01591-14
发表时间：
2015-02-19
期刊：
Genome announcements
影响因子：
0
作者：
Poehlein A;Grosse-Honebrink A;Zhang Y;Minton NP;Daniel R
通讯作者：
Daniel R

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

DOI：
10.1016/j.ymben.2017.01.009
发表时间：
2017-03
期刊：
Metabolic engineering
影响因子：
8.4
作者：
Schwarz KM;Grosse-Honebrink A;Derecka K;Rotta C;Zhang Y;Minton NP
通讯作者：
Minton NP

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants.

DOI：
10.1016/j.anaerobe.2017.09.001
发表时间：
2017-12
期刊：
Anaerobe
影响因子：
2.3
作者：
Grosse-Honebrink A;Schwarz KM;Wang H;Minton NP;Zhang Y
通讯作者：
Zhang Y

A roadmap for gene system development in Clostridium.

DOI：
10.1016/j.anaerobe.2016.05.011
发表时间：
2016-10
期刊：
Anaerobe
影响因子：
2.3
作者：
Minton NP;Ehsaan M;Humphreys CM;Little GT;Baker J;Henstra AM;Liew F;Kelly ML;Sheng L;Schwarz K;Zhang Y
通讯作者：
Zhang Y

Closed Genome Sequence of Clostridium pasteurianum ATCC 6013.