Regulation of R-loops for transcriptional control

用于转录控制的 R 环调节

• 批准号: BB/L009714/1
• 负责人: Caroline Dean
• 金额: $ 67万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL009714%2F1
• 关键词: Regulation; loops; transcriptional; control

The Dean group study the control of flowering time in the reference plant, thalecress (Arabidopsis thaliana). Several flowering pathways converge to regulate a gene called FLC and variation in expression of this gene contributes enormously to natural variation in flowering time of Arabidopsis types collected from around the world. One pathway regulating FLC involves so called non-coding RNAs - for FLC this is the RNA encoded from the opposite strand to the protein. This RNA has been called COOLAIR because it is up regulated by cold temperature. Analysis of the regulation of COOLAIR has recently led to the identification of a novel regulatory mechanism that involves an opening up of the DNA duplex, invasion by RNA and stabilization of this structure by a protein called AtNDX. This structure is called an R-loop and it inhibits production of RNA initiating in that region of the gene. This proposal aims to further dissect this mechanism and identify other proteins that play a role. In the last few years similar non-coding RNAs have been found in many organisms but their regulation and function is generally not known. Advancing our understanding from a study such as this can provide concepts important to gene regulation across many genomes.

Dean小组研究了参比植物拟南芥(Arabiopsis Thaliana)对开花时间的控制。几条开花途径汇聚在一起，调节一种名为FLC的基因，该基因表达的差异极大地影响了从世界各地收集的拟南芥类型的开花时间的自然变异。调节FLC的一个途径涉及到所谓的非编码RNA--对于FLC来说，这是从相反链到蛋白质编码的RNA。这种RNA被称为COOLAIR，因为它受到低温的上调。最近，对COOLAIR调控的分析导致了一种新的调控机制的确定，该机制涉及DNA双链的开放、RNA的入侵和一种名为AtNDX的蛋白质对这种结构的稳定。这种结构被称为R-环，它抑制在该基因区域启动的RNA的产生。这项提议旨在进一步剖析这一机制，并确定其他发挥作用的蛋白质。在过去的几年里，在许多生物体中发现了类似的非编码RNA，但它们的调控和功能通常尚不清楚。从这样的研究中推进我们的理解，可以为许多基因组的基因调控提供重要的概念。

项目成果

R-loop resolution promotes co-transcriptional chromatin silencing.

• DOI: 10.1038/s41467-021-22083-6
• 发表时间: 2021-03-19
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Xu C;Wu Z;Duan HC;Fang X;Jia G;Dean C
• 通讯作者: Dean C

Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.

• DOI: 10.1016/j.celrep.2022.111607
• 发表时间: 2022-11-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Fiedler M;Franco-Echevarría E;Schulten A;Nielsen M;Rutherford TJ;Yeates A;Ahsan B;Dean C;Bienz M
• 通讯作者: Bienz M

Autonomous Pathway: FLOWERING LOCUS C Repression through an Antisense-Mediated Chromatin-Silencing Mechanism

自主途径：通过反义介导的染色质沉默机制抑制开花位点 C

• DOI: 10.1104/pp.19.01009
• 发表时间: 2020-01-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Wu, Zhe;Fang, Xiaofeng;Deati, Caroline
• 通讯作者: Deati, Caroline

Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output.

• DOI: 10.1073/pnas.2113757118
• 发表时间: 2021-11-23
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Xu C;Fang X;Lu T;Dean C
• 通讯作者: Dean C

Plant vernalization proteins contain unusual PHD superdomains without histone H3 binding activity.

• DOI: 10.1016/j.jbc.2022.102540
• 发表时间: 2022-11
• 期刊: The Journal of biological chemistry
• 作者: Franco-Echevarría E;Rutherford TJ;Fiedler M;Dean C;Bienz M
• 通讯作者: Bienz M