Natural variation in vernalization response of Arabidopsis accessions

拟南芥种质春化反应的自然变异

• 批准号: BB/E009662/1
• 负责人: Caroline Dean
• 金额: $ 80.22万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE009662%2F1
• 关键词: Natural; variation; vernalization; response; Arabidopsis

The Dean laboratory is investigating the molecular control of flowering. We use the excellent genetic system of Arabidopsis thaliana as it is easy to clone genes controlling many processes based only on having a mutation (a lesion in the gene). We have focused on why and how prolonged cold influences flowering, a process called vernalization. In nature, plants experience prolonged cold during winter and many plants do not flower until they have experienced this prolonged cold. This is an adaptation to ensure flowering in spring when conditions are favourable for maximal flower production and seed set. Many genes controlling this process have now been cloned so in this proposal we want to investigate how their function has changed during adaptation of Arabidopsis to different climates. We are working with Arabidopsis accessions collected from a range of latitudes that extend up to the edge of the Arctic Circle. These plants need much longer periods of cold to accelerate flowering than ones collected from the UK. We have made crosses between these different plants and have analysed the flowering and vernalization response of their progeny. This has indicated that the natural variation in vernalization is the result of allelic variation of 4-5 genes. We now want to identify which genes these are, understand what changes have occurred and how those affect the flowering behaviour. In collaboration with experts in population and ecological genetics, we also want to determine if those changes have spread in the worldwide Arabidopsis population and understand how much advantage those changes confer to the plants in the different environmental conditions.

迪安实验室正在研究开花的分子控制。我们利用拟南芥优良的遗传系统，因为它很容易克隆控制许多过程的基因，仅基于突变（基因中的病变）。我们关注的是长时间的寒冷影响开花的原因和方式，这一过程被称为春化。在自然界中，植物在冬天经历了长时间的寒冷，许多植物在经历了长时间的寒冷之后才开花。这是一种适应，以确保在春天开花，因为春天的条件有利于最大的开花和结实率。许多控制这一过程的基因现在已经被克隆，因此在本提案中，我们想研究它们的功能在拟南芥适应不同气候的过程中是如何变化的。我们正在研究从一系列纬度地区收集的拟南芥，这些纬度一直延伸到北极圈的边缘。这些植物比从英国收集的植物需要更长的寒冷期来加速开花。我们对这些不同的植物进行了杂交，并分析了它们后代的开花和春化反应。这表明春化的自然变异是4-5个基因等位变异的结果。我们现在想要确定这些是哪些基因，了解发生了什么变化以及这些变化是如何影响开花行为的。通过与种群和生态遗传学专家的合作，我们还想确定这些变化是否已经在全世界的拟南芥种群中传播，并了解这些变化在不同的环境条件下给植物带来了多少优势。

项目成果

The PHD finger protein VRN5 functions in the epigenetic silencing of Arabidopsis FLC

• DOI: 10.1016/j.cub.2006.11.052
• 发表时间: 2007-01-09
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Greb, Thomas;Mylne, Joshua S.;Dean, Caroline
• 通讯作者: Dean, Caroline

Differential interactions of the autonomous pathway RRM proteins and chromatin regulators in the silencing of Arabidopsis targets.

• DOI: 10.1371/journal.pone.0002733
• 发表时间: 2008-07-16
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Bäurle I;Dean C
• 通讯作者: Dean C

VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC.

• DOI: 10.1038/s41467-022-32897-7
• 发表时间: 2022-09-21
• 期刊: Nature communications
• 影响因子: 16.6

Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.

• DOI: 10.1016/j.celrep.2022.111607
• 发表时间: 2022-11-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Fiedler M;Franco-Echevarría E;Schulten A;Nielsen M;Rutherford TJ;Yeates A;Ahsan B;Dean C;Bienz M
• 通讯作者: Bienz M

Multiple FLC haplotypes defined by independent cis-regulatory variation underpin life history diversity in Arabidopsis thaliana.

• DOI: 10.1101/gad.245993.114
• 发表时间: 2014-08-01
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Li P;Filiault D;Box MS;Kerdaffrec E;van Oosterhout C;Wilczek AM;Schmitt J;McMullan M;Bergelson J;Nordborg M;Dean C
• 通讯作者: Dean C