Epigenetic reprogramming of FLC

FLC的表观遗传重编程

• 批准号: BB/G009562/1
• 负责人: Caroline Dean
• 金额: $ 57.38万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG009562%2F1
• 关键词: Epigenetic; reprogramming; FLC

The Dean laboratory has been investigating what controls the timing of flowering. We are using a model plant Arabidopsis thaliana to identify genes involved in controlling flowering as in this plant it is easy to clone genes based on a mutant phenotype. Over the years we have shown that a key player in the regulation of flowering is a protein called FLC. FLC prevents flowering by stopping the activation of a set of genes needed to make flowers. FLC expression levels are reduced by prolonged cold and then remain stably off through the rest of the life-cycle of that plant. This stable repression is caused by chromatin silencing of the FLC gene through a conserved mechanism involving Polycomb group proteins. Before the next generation the levels of this gene need to be reset to ensure the progeny also need vernalization for flowering. This process may have many parallels with epigenetic reprogramming in mammals / the process that went wrong in the cloned sheep Dolly. We have mutations that disrupt the reprogramming of FLC and wish to exploit these to identify the molecular mechanisms involved. We will explore any parallels between FLC resetting and the epigenetic reprogramming that occurs in the mouse germline through a collaboration with Prof. Azim Surani in Cambridge. Our work on FLC epigenetic reprogramming is likely to produce concepts relevant to gene regulation throughout biology.

迪恩实验室一直在调查是什么控制了开花的时间。我们正在使用模式植物拟南芥来鉴定控制开花的基因，因为在这种植物中，很容易根据突变的表型来克隆基因。多年来，我们已经证明，在开花调控中起关键作用的是一种名为FLC的蛋白质。FLC通过停止开花所需的一组基因的激活来阻止开花。FLC的表达水平会因长时间的寒冷而降低，然后在该植物的其余生命周期中保持稳定。这种稳定的抑制是由染色质沉默FLC基因引起的，这是一种涉及多梳蛋白的保守机制。在下一代之前，需要重新设置该基因的水平，以确保后代也需要春化才能开花。这一过程可能与哺乳动物的表观遗传重新编程/克隆绵羊多莉出错的过程有许多相似之处。我们有破坏FLC重新编程的突变，并希望利用这些突变来确定相关的分子机制。我们将通过与剑桥的Azim Surani教授合作，探索FLC重置和小鼠胚系中发生的表观遗传学重编程之间的任何相似之处。我们在FLC表观遗传重编程方面的工作可能会产生与整个生物学中的基因调控相关的概念。

项目成果

Publisher Correction: Temperature-dependent growth contributes to long-term cold sensing.

出版商更正：温度依赖性生长有助于长期冷感。

• DOI: 10.1038/s41586-020-2694-x
• 发表时间: 2020
• 期刊: Nature
• 影响因子: 64.8
• 作者: Zhao Y

Plant vernalization proteins contain unusual PHD superdomains without histone H3 binding activity.

• DOI: 10.1016/j.jbc.2022.102540
• 发表时间: 2022-11
• 期刊: The Journal of biological chemistry
• 作者: Franco-Echevarría E;Rutherford TJ;Fiedler M;Dean C;Bienz M
• 通讯作者: Bienz M

Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.

• DOI: 10.1016/j.celrep.2022.111607
• 发表时间: 2022-11-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Fiedler M;Franco-Echevarría E;Schulten A;Nielsen M;Rutherford TJ;Yeates A;Ahsan B;Dean C;Bienz M
• 通讯作者: Bienz M

VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC.

• DOI: 10.1038/s41467-022-32897-7
• 发表时间: 2022-09-21
• 期刊: Nature communications
• 影响因子: 16.6

Epigenetic reprogramming that prevents transgenerational inheritance of the vernalized state.

• DOI: 10.1038/nature13722
• 发表时间: 2014-11-27
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Crevillen, Pedro;Yang, Hongchun;Cui, Xia;Greeff, Christiaan;Trick, Martin;Qiu, Qi;Cao, Xiaofeng;Dean, Caroline
• 通讯作者: Dean, Caroline