Mechanistic and evolutionary analysis of the natural variation in Arabidopsis vernalization response

拟南芥春化反应自然变异的机制和进化分析

• 批准号: BB/I007857/1
• 负责人: Caroline Dean
• 金额: $ 92.01万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI007857%2F1
• 关键词: Mechanistic; evolutionary; analysis; natural; variation

A full understanding of how climate change will influence plants requires an understanding of how mechanisms used to monitor environmental cues have changed as plants have adapted to different habitats. Regulation of flowering in many plants in temperate and arctic environments includes a requirement for a period of exposure to cold, known as vernalization. In this proposal we want to fully characterize the molecular basis of natural variation in vernalization. A major regulator of this process is FLC, a floral repressor. The gene encoding FLC needs to be silenced for flowering to proceed and the speed this happens differs in Arabidopsis collected from different habitats. We will analyse FLC regulation and how it has evolved in response to adaptation to different climates. 'Mix-and-match' versions of FLC genes from the different natural variants will be analysed in transgenic plants to uncover which of the different nucleotide changes contributes to the altered vernalization response. In collaboration with Dr Magnus Nordborg, a population geneticist, we will then analyse FLC in a worldwide set of Arabidopsis accessions and relate sequence variation to vernalization response. We will also undertake experiments analysing the fitness benefits of this variation and compare these data to field-derived phenotypic data generated in two parallel studies. This will reveal whether variation in vernalization is important for adaptation.

要充分了解气候变化将如何影响植物，需要了解用于监测环境线索的机制如何随着植物适应不同的栖息地而发生变化。在温带和北极环境中，许多植物对开花的调节包括一段时间暴露在寒冷中，被称为春化。在这个建议中，我们想要充分表征春化自然变异的分子基础。这一过程的主要调节者是FLC，一种花抑制因子。编码FLC的基因需要沉默才能开花，而在不同栖息地采集的拟南芥中，这种情况发生的速度是不同的。我们将分析FLC的调节，以及它是如何进化以适应不同的气候。来自不同自然变异的FLC基因的“混合和匹配”版本将在转基因植物中进行分析，以揭示哪些不同的核苷酸变化导致了春化反应的改变。在与种群遗传学家Magnus Nordborg博士的合作下，我们将分析世界范围内一系列拟南芥的FLC，并将序列变异与春化反应联系起来。我们还将进行实验，分析这种变异的适应度效益，并将这些数据与两个平行研究中产生的现场衍生表型数据进行比较。这将揭示春化的变异是否对适应很重要。

项目成果

Phenotypic evolution through variation in splicing of the noncoding RNA COOLAIR.

• DOI: 10.1101/gad.258814.115
• 发表时间: 2015-04-01
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Li P;Tao Z;Dean C
• 通讯作者: Dean C

VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC.

• DOI: 10.1038/s41467-022-32897-7
• 发表时间: 2022-09-21
• 期刊: Nature communications
• 影响因子: 16.6

Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.

• DOI: 10.1016/j.celrep.2022.111607
• 发表时间: 2022-11-08
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Fiedler M;Franco-Echevarría E;Schulten A;Nielsen M;Rutherford TJ;Yeates A;Ahsan B;Dean C;Bienz M
• 通讯作者: Bienz M

Molecular variation in a functionally divergent homolog of FCA regulates flowering time in Arabidopsis thaliana.

FCA 功能不同同源物的分子变异调节拟南芥开花时间

• DOI: 10.1038/s41467-020-19666-0
• 发表时间: 2020-11-17
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Wang Y;Tao Z;Wang W;Filiault D;Qiu C;Wang C;Wang H;Rehman S;Shi J;Zhang Y;Li P
• 通讯作者: Li P

Major-effect alleles at relatively few loci underlie distinct vernalization and flowering variation in Arabidopsis accessions.

• DOI: 10.1371/journal.pone.0019949
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Strange A;Li P;Lister C;Anderson J;Warthmann N;Shindo C;Irwin J;Nordborg M;Dean C
• 通讯作者: Dean C