BIOEFFECTS OF DIAGNOSTIC ULTRASOUND RADIATION

诊断超声辐射的生物效应

• 批准号: 3182362
• 负责人: PETER D EDMONDS
• 金额: $ 22.68万
• 依托单位: SRI INTERNATIONAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3182362
• 关键词: biomedical equipment safety; diagnosis procedure safety; laboratory mouse; macrophage; membrane activity; membrane structure; noninvasive diagnosis; radiation hazard; radiobiology; radiodiagnosis; sheep; ultrasonography

The objective of this study is to determine whether ultrasound exposure results in measurable alterations of cell membrane structures or membrane functions when applied to suspensions of mouse-derived peritoneal cells. The presence of ultrasound- induced cellular aberrations will be determined on purified macrophage populations using in vitro culture assays designed to detect alterations in cell surface determinants and functions characteristic of the macrophage. The broader objectives of this proposed study of bioeffects of pulsed ultrasound clinical diagnostic units are: o To enhance knowledge of the effects of ultrasound on the cell membrane through study of a variety of immunological endpoints. o To aid in assessing the risk (if any) of continued use of ultrasound in clinical diagnosis. Specific aims leading to these objectives are: o To identify and substantiate positive or negative results in the cases of a variety of immunological endpoints, some of which are previously unused in this field of research. o To determine, as far as possible, the mechanism of interaction of ultrasound with cells in suspension that give rise to any of the positive results. The following endpoints will be used: adherence and spreading; expression of Fc and C3b receptors; phagocytic index for sheep red blood cells; phagocytosis and intracellular killing of S. aureus; cytolytic activity of activated macrophages against murine tumor cells; ability to serve as accessory cells during in vitro mitogen stimulation of murine derived T-lymphocytes. During the study, we expect to accomplish: o Replicative assays by six methods utilizing nine sets of ultrasound exposure conditions that span a wide but typical range of exposure parameters. o Identification of endpoints that yield positive and negative results with customary statistical levels of confidence. o A delineation of the limits of exposure parameters that produce positive results (if any). o A set of experiments indicating the mechanism(s) of interaction of ultrasound with the cell membrane that result in immunological effects (if any).

本研究的目的是确定超声是否 暴露导致细胞膜的可测量的改变 结构或膜功能时，适用于悬浮液 小鼠腹膜细胞。 超声波的出现- 诱导的细胞畸变将在纯化的 巨噬细胞群体，使用体外培养测定， 检测细胞表面决定簇和功能的改变 巨噬细胞的特征。 这项拟议的生物效应研究的更广泛目标是 脉冲超声临床诊断单元是： o提高对超声波对细胞影响的认识 通过研究各种免疫学终点， o帮助评估继续使用的风险（如有） 超声在临床诊断中的应用 实现这些目标的具体目标是： o确定和证实积极或消极的结果， 各种免疫学终点的病例，其中一些是 以前在该研究领域未使用过。 尽可能确定相互作用的机制 超声波与悬浮细胞的混合， 积极的结果。 将使用以下终点：依从性和扩散; Fc和C3 b受体的表达;绵羊的吞噬指数 红细胞; S.金黄色; 活化巨噬细胞对小鼠肿瘤细胞的杀伤作用 细胞;在体外有丝分裂原中作为辅助细胞的能力 刺激鼠源性T淋巴细胞。 在研究过程中，我们希望实现： o通过六种方法使用九组重复测定， 超声波暴露条件跨越广泛但典型的范围， 曝光参数。 o确定产生积极和消极影响的终点 结果具有常规统计置信水平。 o描述产生以下结果的暴露参数的限值 阳性结果（如有）。 o一组表明相互作用机制的实验 超声波对细胞膜的作用， 影响（如有）。

项目成果

Conservation of bactericidal activity in ultrasound-exposed murine peritoneal phagocytic cells.

超声暴露的小鼠腹膜吞噬细胞杀菌活性的保存。

• DOI: 10.1016/0301-5629(92)90074-k
• 发表时间: 1992
• 期刊: Ultrasound in medicine & biology
• 影响因子: 2.9
• 作者: Prohaska,SS;Rimer,VG;Tusé,D;Pryor,GT;Edmonds,PD
• 通讯作者: Edmonds,PD

Conservation of receptor expression and phagocytic activity of murine macrophages exposed to various ultrasonic regimens in vitro.

体外暴露于各种超声方案的小鼠巨噬细胞的受体表达和吞噬活性的保护。

• DOI: 10.7863/jum.1992.11.2.93
• 发表时间: 1992
• 期刊: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
• 作者: Prohaska,SS;Rimer,VG;Winters,MA;Humphres,RC;Tusé,D;Edmonds,PD
• 通讯作者: Edmonds,PD