BIOEFFECTS OF DIAGNOSTIC ULTRASOUND RADIATION

诊断超声辐射的生物效应

• 批准号: 3182361
• 负责人: PETER D EDMONDS
• 金额: $ 22.37万
• 依托单位: SRI INTERNATIONAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3182361
• 关键词: biomedical equipment safety; diagnosis procedure safety; laboratory mouse; macrophage; membrane activity; membrane structure; noninvasive diagnosis; radiation hazard; radiobiology; radiodiagnosis; sheep; ultrasonography

The objective of this study is to determine whether ultrasound exposure results in measurable alterations of cell membrane structures or membrane functions when applied to suspensions of mouse-derived peritoneal cells. The presence of ultrasound- induced cellular aberrations will be determined on purified macrophage populations using in vitro culture assays designed to detect alterations in cell surface determinants and functions characteristic of the macrophage. The broader objectives of this proposed study of bioeffects of pulsed ultrasound clinical diagnostic units are: o To enhance knowledge of the effects of ultrasound on the cell membrane through study of a variety of immunological endpoints. o To aid in assessing the risk (if any) of continued use of ultrasound in clinical diagnosis. Specific aims leading to these objectives are: o To identify and substantiate positive or negative results in the cases of a variety of immunological endpoints, some of which are previously unused in this field of research. o To determine, as far as possible, the mechanism of interaction of ultrasound with cells in suspension that give rise to any of the positive results. The following endpoints will be used: adherence and spreading; expression of Fc and C3b receptors; phagocytic index for sheep red blood cells; phagocytosis and intracellular killing of S. aureus; cytolytic activity of activated macrophages against murine tumor cells; ability to serve as accessory cells during in vitro mitogen stimulation of murine derived T-lymphocytes. During the study, we expect to accomplish: o Replicative assays by six methods utilizing nine sets of ultrasound exposure conditions that span a wide but typical range of exposure parameters. o Identification of endpoints that yield positive and negative results with customary statistical levels of confidence. o A delineation of the limits of exposure parameters that produce positive results (if any). o A set of experiments indicating the mechanism(s) of interaction of ultrasound with the cell membrane that result in immunological effects (if any).

本研究的目的是确定超声是否 暴露导致细胞膜发生可测量的变化 应用于悬浮液时的结构或膜功能 小鼠来源的腹膜细胞。 超声波的存在—— 诱导的细胞畸变将在纯化后测定 使用体外培养测定的巨噬细胞群旨在 检测细胞表面决定因素和功能的变化 巨噬细胞的特征。 这项拟议的生物效应研究的更广泛目标 脉冲超声临床诊断单位有： o 加深对超声波对细胞影响的了解 通过研究各种免疫学终点来研究膜。 o 帮助评估继续使用的风险（如果有） 超声在临床诊断中的应用。 实现这些目标的具体目标是： o 识别并证实积极或消极的结果 多种免疫学终点的病例，其中一些是 以前在该研究领域未使用过。 o 尽可能确定相互作用的机制 超声波与悬浮细胞产生任何 积极的结果。 将使用以下终点：坚持和传播； Fc 和 C3b 受体的表达；羊吞噬指数 红细胞；金黄色葡萄球菌的吞噬作用和细胞内杀伤作用； 活化巨噬细胞对小鼠肿瘤的细胞溶解活性 细胞；在体外有丝分裂过程中充当辅助细胞的能力 刺激小鼠来源的 T 淋巴细胞。 在研究过程中，我们期望实现： o 使用九组的六种方法进行重复测定 超声波暴露条件跨越广泛但典型的范围 曝光参数。 o 识别产生积极和消极的终点 结果具有惯常的统计置信水平。 o 描述产生的暴露参数的限制 积极的结果（如果有的话）。 o 一组表明相互作用机制的实验 超声波作用于细胞膜，导致免疫学 影响（如果有的话）。