BIOEFFECTS OF DIAGNOSTIC ULTRASOUND RADIATION

诊断超声辐射的生物效应

• 批准号: 3182360
• 负责人: PETER D EDMONDS
• 金额: $ 21.53万
• 依托单位: SRI INTERNATIONAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3182360
• 关键词: biomedical equipment safety; diagnosis procedure safety; laboratory mouse; macrophage; membrane activity; membrane structure; noninvasive diagnosis; radiation hazard; radiobiology; radiodiagnosis; sheep; ultrasonography

The objective of this study is to determine whether ultrasound exposure results in measurable alterations of cell membrane structures or membrane functions when applied to suspensions of mouse-derived peritoneal cells. The presence of ultrasound- induced cellular aberrations will be determined on purified macrophage populations using in vitro culture assays designed to detect alterations in cell surface determinants and functions characteristic of the macrophage. The broader objectives of this proposed study of bioeffects of pulsed ultrasound clinical diagnostic units are: o To enhance knowledge of the effects of ultrasound on the cell membrane through study of a variety of immunological endpoints. o To aid in assessing the risk (if any) of continued use of ultrasound in clinical diagnosis. Specific aims leading to these objectives are: o To identify and substantiate positive or negative results in the cases of a variety of immunological endpoints, some of which are previously unused in this field of research. o To determine, as far as possible, the mechanism of interaction of ultrasound with cells in suspension that give rise to any of the positive results. The following endpoints will be used: adherence and spreading; expression of Fc and C3b receptors; phagocytic index for sheep red blood cells; phagocytosis and intracellular killing of S. aureus; cytolytic activity of activated macrophages against murine tumor cells; ability to serve as accessory cells during in vitro mitogen stimulation of murine derived T-lymphocytes. During the study, we expect to accomplish: o Replicative assays by six methods utilizing nine sets of ultrasound exposure conditions that span a wide but typical range of exposure parameters. o Identification of endpoints that yield positive and negative results with customary statistical levels of confidence. o A delineation of the limits of exposure parameters that produce positive results (if any). o A set of experiments indicating the mechanism(s) of interaction of ultrasound with the cell membrane that result in immunological effects (if any).

这项研究的目的是确定超声波是否 暴露可导致细胞膜的可测量变化 应用于悬浮液时的结构或膜功能 小鼠来源的腹膜细胞。超声波的存在- 诱导的细胞畸变率将在纯化后测定 巨噬细胞群体使用体外培养试验设计 检测细胞表面决定因素和功能的变化 巨噬细胞的特征。 这项拟议的生物效应研究的更广泛的目标是 脉冲超声临床诊断单元有： O加强对超声波对细胞影响的认识 通过对细胞膜的各种免疫终点的研究。 O协助评估继续使用的风险(如有的话) 超声在临床诊断中的应用。 实现这些目标的具体目标是： O以确定和证实在 各种免疫终点的病例，其中一些是 以前在这一研究领域中没有使用过的。 O尽可能确定相互作用的机制 超声波与悬浮液中的细胞一起产生任何 结果是积极的。 将使用以下端点：坚持和传播； Fc和C3b受体的表达；绵羊的吞噬指数 红细胞；金黄色葡萄球菌的吞噬和胞内杀灭； 活化巨噬细胞对小鼠肿瘤的杀伤作用 细胞；在体外有丝分裂原中充当辅助细胞的能力 对小鼠源性T淋巴细胞的刺激作用。 在研究期间，我们期望实现以下目标： O采用六种方法进行复制分析，利用九套 超声暴露条件跨越广泛但典型的范围 曝光参数。 O确定产生正值和负值的端点 结果具有惯常的统计置信度水平。 O划定产生以下结果的曝光参数的限度 积极的结果(如果有)。 O一组表明相互作用机理的实验(S) 超声波与细胞膜的相互作用，导致免疫学 影响(如有)。