13 ERA-CAPS: Functional characterisation and validation of nonhost components in Triticeae species for durable resistance against fungal diseases

13 ERA-CAPS：小麦科物种非寄主成分的功能表征和验证，以持久抵抗真菌病害

• 批准号: BB/M000710/1
• 负责人: Pietro Spanu
• 金额: $ 43.5万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM000710%2F1
• 关键词: 13; ERA; CAPS; Functional; characterisation

Nonhost resistance (NHR) is the most durable and broadly acting form of resistance plants posses to ward off the majority of pathogen attacks regularly occurring within the environment they are living in. In order to exploit NHR in future crop protection concepts we need to understand why the minority of adapted host pathogens can circumvent or suppress NHR and what defense- or accommodation-related proteins, signalling pathways or structural components of their host plants are targeted in order to establish disease. A major obstacle to the rapid exploitation of NHR in crop breeding is related to the fact that NHR is operating at the species level, and only in exceptional cases corresponding sources of resistance can be crossed with related crop plants. Therefore, gene technological approaches to transfer nonhost-resistance components across species barriers are an attractive alternative to traditional or molecular breeding.In the ERA-NET consortium TritNONHOST, plus a number of related projects e.g. funded within the German GABI program (BMBF), we identified a number of genes and genetic loci in barley and wheat that are associated or correlated with NHR to three major fungal pathogens including powdery mildews. A limited set of those belonging to the group of receptor-like kinases were successfully validated in functional transient assays in barley and wheat and will provide an important source for the proposed work in the DURESTrit consortium, in addition to resistance loci derived from the wild barley species Hordeum bulbosum or from experimental barley populations segregating for NHR strength. In DURESTrit, we propose to functionally validate receptor-like kinase genes plus a limited number of genes with outstanding regulation behaviour in host- versus nonhost interactions both in barley and wheat by generating and characterizing stable transgenic and backcross lines from wild barley introgressions, respectively. These materials will either carry transgenes or genome fragments from NHR donors, or have potentially important resistance components silenced by RNAi or genetically modified using TALEN technologyThe project will result in the validation of previously identified strong NHR candidates , with a special emphasis on receptor-like kinases, and in the identification of new NHR components introduced into barley or wheat by wide crosses or genetic engineering. This will deepen our understanding of NHR in cereals and provide materials and know-how for the exploitation of NHR by translational research.

非寄主抗性（NHR）是植物抵抗大多数病原体攻击的最持久和最广泛的抗性形式。为了在未来的作物保护概念中利用NHR，我们需要了解为什么少数适应的宿主病原体可以规避或抑制NHR，以及它们的宿主植物的防御或防御相关蛋白质，信号传导途径或结构成分是为了建立疾病。在作物育种中快速利用NHR的一个主要障碍与NHR在物种水平上运作的事实有关，并且仅在例外情况下，相应的抗性来源可以与相关的作物植物杂交。因此，通过基因技术跨越物种屏障转移非寄主抗性组分是传统或分子育种的一种有吸引力的替代方法。在ERA-NET联盟TritNONHOST中，加上一些相关项目，例如德国GABI计划（BMBF）资助的项目，我们在大麦和小麦中鉴定了一些与NHR相关的基因和遗传位点，这些NHR与三种主要的真菌病原体有关，粉状霉菌一组有限的那些属于受体样激酶组的成功地验证了在大麦和小麦的功能性瞬时测定，并将提供一个重要的来源，拟议的工作在DURESTrit财团，除了来自野生大麦种大麦或实验大麦种群分离NHR强度的抗性位点。在DURESTrit中，我们建议功能验证受体样激酶基因加上数量有限的基因在大麦和小麦中的宿主与非宿主相互作用中具有出色的调节行为，分别通过产生和表征野生大麦基因渗入的稳定转基因和回交系。这些材料将携带来自NHR供体的转基因或基因组片段，或者具有潜在的重要抗性组分，这些抗性组分通过RNAi沉默或使用TALEN技术进行遗传修饰。该项目将导致对先前确定的强NHR候选物的验证，特别强调受体样激酶，并通过远缘杂交或遗传工程鉴定引入大麦或小麦的新NHR组分。这将加深我们对谷物中NHR的理解，并为NHR的转化研究提供材料和技术。

项目成果

The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA

真菌核糖核酸酶样效应蛋白 CSEP0064/BEC1054 抑制植物免疫并干扰宿主核糖体 RNA 的降解

• DOI: 10.18154/rwth-2019-02738
• 发表时间: 2019
• 作者: Pennington H

Signatures of host specialization and a recent transposable element burst in the dynamic one-speed genome of the fungal barley powdery mildew pathogen.

• DOI: 10.1186/s12864-018-4750-6
• 发表时间: 2018-05-22
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Frantzeskakis L;Kracher B;Kusch S;Yoshikawa-Maekawa M;Bauer S;Pedersen C;Spanu PD;Maekawa T;Schulze-Lefert P;Panstruga R
• 通讯作者: Panstruga R

Messages from Powdery Mildew DNA: How the Interplay with a Host Moulds Pathogen Genomes

来自白粉病 DNA 的信息：与宿主的相互作用如何塑造病原体基因组

• DOI: 10.1016/s2095-3119(13)60650-4
• 发表时间: 2014
• 期刊: Journal of Integrative Agriculture
• 影响因子: 4.8
• 作者: Pietro D S

Identification and selection of normalization controls for quantitative transcript analysis in Blumeria graminis.

• DOI: 10.1111/mpp.12300
• 发表时间: 2016-05
• 期刊: Molecular plant pathology
• 影响因子: 4.9
• 作者: Pennington HG;Li L;Spanu PD
• 通讯作者: Spanu PD