14TSB_SynBio A High Throughput Miniaturised Mass Spectrometry Tool for Profiling Synthetic Design Libraries

14TSB_SynBio 用于分析合成设计文库的高通量小型化质谱工具

• 批准号: BB/M005577/1
• 负责人: Thomas Ellis
• 金额: $ 11.87万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM005577%2F1
• 关键词: 14TSB_SynBio; Throughput; Miniaturised; Mass; Spectrometry

Over the last 10 years, the new field of synthetic biology has advanced the existing science of genetic modification by applying principles of engineering; design, simulation and testing. This approach has allowed us to predictably create exciting new technologies by modifying safe microbes with customised DNA to perform new tasks including computation, multi-input environmental sensing and efficient production of medicinal drugs and bioenergy, primarily through metabolic pathway engineering. As our ability to write DNA and engineer microbes accelerates, we are becoming limited by the lack of high-data, high-throughput cell measurement methods available with which to assess the performance of customised strains. The project links foundational synthetic biology and DNA assembly expertise at Imperial College London with microfluidics and mass spectrometry expertise at the industrial partner, Sphere Fluidics Limited, in order to build, test and demonstrate a novel microfluidics-mass spectrometry tool that should allow in-depth measurement of the performance of thousands of engineered microbes per run. The intention of the project is to develop an equipment set-up (interfaced microfluidics and mass spectrometry) and a methodology that together is a platform tool with which to characterise collections of thousands of engineered microbe strains. This tool can then be sold downstream as a service (or as an equipment set-up) for companies and institutions involved in synthetic biology.

在过去的十年中，合成生物学的新领域通过应用工程原理推进了现有的基因改造科学；设计，仿真和测试。这种方法使我们能够预见地创造出令人兴奋的新技术，通过使用定制的DNA修改安全的微生物来执行新的任务，包括计算，多输入环境感知和有效生产药物和生物能源，主要是通过代谢途径工程。随着我们编写DNA和工程微生物的能力加快，我们正受到缺乏高数据、高通量细胞测量方法的限制，这些方法可用于评估定制菌株的性能。该项目将伦敦帝国理工学院的基础合成生物学和DNA组装专业知识与工业合作伙伴Sphere Fluidics Limited的微流体和质谱专业知识结合起来，以构建、测试和演示一种新型微流体-质谱工具，该工具应该可以对每次运行的数千个工程微生物的性能进行深入测量。该项目的目的是开发一种设备设置（界面微流体和质谱）和一种方法，它们共同构成一个平台工具，用于表征数千种工程微生物菌株的集合。然后，该工具可以作为服务（或设备设置）出售给涉及合成生物学的公司和机构。