Grow-Your-Own Composites: Programming Diverse Material Properties for Defence into Engineered Bacterial Cellulose

自行种植复合材料：将用于防御的多种材料特性编程到工程细菌纤维素中

• 批准号: EP/N026489/1
• 负责人: Thomas Ellis
• 金额: $ 69.14万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FN026489%2F1
• 关键词: Grow; Your; Own; Composites; Programming

Bacterial cellulose is a strong, ultrapure form of the biomaterial nanocellulose, which is naturally made in large amounts by several species of Acetobacter bacteria including K. rhaeticus. Bacterial cellulose is cheap to produce, has desirable purity, high crystallinity and tensile properties and does not contain other impurities like those found in plant cellulose. It is mouldable, biocompatible and capable of storing water over 90% of its total weight, and has found numerous commercial applications in medical wound-dressings, high-end acoustics, and many other diverse products.In this proposal, we plan to build on our recent success guiding the Imperial College 2014 iGEM team in developing genetic manipulation methods and a synthetic biology toolkit for K. rhaeticus, the first toolkit of note for bacteria that produce cellulose in high yields. Our vision is to use synthetic biology methods to modify the production of bacterial cellulose from K. rhaeticus so that the bacterial cultures now produce programmable cellulose composites that have diverse and highly-desired material properties, ideally for defence applications. By using our synthetic biology tools and expanding this toolkit with further features such as genome editing and light-based control, we will be able to alter and control bacteria at the DNA level so that they now can be made to secrete modified bacterial cellulose with different bulk properties such as altered hydrophobicity. We will also use our toolkit to get our growing bacteria to produce interwoven mixtures of bacterial cellulose and other biomaterials such as bioplastics, functional proteins (e.g. enzymes) and protein polymers (e.g. curli fibres and silks). The result will be a variety of biosynthesised nanocellulose composites, likely to have valuable material properties that improve the strength and ductility of materials fabricated with this substrate, without increasing the weight and cost significantly further. Combining our team's considerable expertise in synthetic biology, composite engineering and blast research, we will together develop methods to safely convert these bacterial cellulose composites into lightweight layered composite materials and into advanced aerogels that match the material properties desired for defence applications in protection and shock absorption and more. We will test the mechanical properties of our new biosynthesised composites and use this to feedback to improved second-generation designs. Our project brings together Synthetic Biology and Advanced Materials, two of the UK's Eight Great Technologies, and will lay the foundations for using DNA-based engineering of cells to produce advanced biomaterial composites with many diverse and valuable future applications.

细菌纤维素是一种强大的、超纯的生物材料纳米纤维素，它是由包括Rhaeticus在内的几种醋酸杆菌自然大量合成的。细菌纤维素的生产成本低，具有理想的纯度、高结晶度和拉伸性能，并且不含植物纤维素中的其他杂质。它可模塑，生物兼容，能够储存超过其总重量90%的水，并已在医疗伤口敷料、高端声学和许多其他不同产品中获得了大量商业应用。在这项提案中，我们计划在最近成功指导帝国理工学院2014年iGEM团队开发遗传操作方法和K.rhaeticus合成生物学工具包的基础上再接再厉，Khaeticus是第一个值得注意的高产纤维素细菌工具包。我们的愿景是使用合成生物学方法来修改从Khaeticus生产的细菌纤维素，以便细菌培养现在生产具有多种和高度期望的材料特性的可编程纤维素复合材料，理想地用于国防应用。通过使用我们的合成生物学工具，并将该工具包扩展为进一步的功能，如基因组编辑和基于光的控制，我们将能够在DNA水平上改变和控制细菌，以便现在可以使它们分泌具有不同体积性质的改良细菌纤维素，例如改变疏水性。我们还将使用我们的工具包让我们正在生长的细菌生产细菌纤维素和其他生物材料的交织混合物，如生物塑料、功能蛋白质(例如酶)和蛋白质聚合物(例如卷曲纤维和丝绸)。其结果将是各种生物合成的纳米纤维素复合材料，可能具有有价值的材料特性，改善用这种基材制造的材料的强度和延展性，而不会进一步显著增加重量和成本。结合我们团队在合成生物学、复合材料工程和爆炸研究方面的丰富专业知识，我们将共同开发安全地将这些细菌纤维素复合材料转化为轻质层状复合材料和先进气凝胶的方法，以满足国防保护和减震等方面所需的材料性能要求。我们将测试我们新的生物合成复合材料的机械性能，并将其反馈给改进的第二代设计。我们的项目将合成生物学和先进材料结合在一起，这是英国八大技术中的两项，并将为使用基于DNA的细胞工程来生产具有许多不同和有价值的未来应用的先进生物材料复合材料奠定基础。

项目成果

On the BET Surface Area of Nanocellulose Determined Using Volumetric, Gravimetric and Chromatographic Adsorption Methods

• DOI: 10.3389/fceng.2021.738995
• 发表时间: 2021-09
• 作者: A. Kondor;Alba Santmarti;A. Mautner;Daryl R. Williams;A. Bismarck;Koon-Yang Lee

Increasing bacterial cellulose compression resilience with glycerol or PEG400 for robuster engineered living materials.

使用甘油或 PEG400 提高细菌纤维素的压缩弹性，以获得更坚固的工程活性材料。

• DOI: 10.1016/j.carpta.2022.100245
• 发表时间: 2022
• 期刊: Carbohydrate Polymer Technologies and Applications
• 影响因子: 5.5
• 作者: Caro-Astorga J

Increasing Bacterial Cellulose Compression Resilience with Glycerol or Peg400 for Robuster Engineered Living Materials

使用甘油或 Peg400 提高细菌纤维素压缩弹性，用于坚固的工程活性材料

• DOI: 10.2139/ssrn.4079357
• 发表时间: 2022
• 期刊: SSRN Electronic Journal
• 作者: Caro-Astorga J

Bacterial cellulose spheroids as building blocks for 2D and 3D engineered living materials

细菌纤维素球体作为 2D 和 3D 工程生活材料的构建模块

• DOI: 10.1101/2020.05.11.088138
• 发表时间: 2020
• 作者: Caro-Astorga J

Tough poly(ethylene glycol)-sized bacterial cellulose sheet for high impact strength laminated acrylic composites

用于高冲击强度层压丙烯酸复合材料的坚韧聚乙二醇尺寸细菌纤维素片

• DOI: 10.1016/j.compositesa.2022.106845
• 发表时间: 2022
• 期刊: Applied Science and Manufacturing
• 作者: Herrera N