Utilising Illumina sequencing for high throughput genotyping of wheat

利用 Illumina 测序对小麦进行高通量基因分型

• 批准号: BB/P004857/1
• 负责人: Michael Bevan
• 金额: $ 25.34万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FP004857%2F1
• 关键词: Utilising; Illumina; sequencing; throughput; genotyping

Genetic markers are used to identify differences in the genetic make-up of individuals in large populations. In crop plants genetic markers are very important for breeding improved crops because they can be used to trace the contributions of parents to their offspring. This enable breeders to identify quickly regions of DNA that confer desirable traits and breed these regions into lines, while eliminating undesired regions. Crop breeding requires many plants to be screened for their genetic make-up over several years. The more genetic markers that are used the more precise breeding can be made, reducing the time and uncertainty in the process. Achieving improved efficiency and predictability in plant breeding is centrally important for securing future supplies of key crops such as wheat. We aim to use our expertise in wheat genomics and next-generation sequencing to develop high- throughput genotyping for wheat in collaboration with UK wheat breeders.

遗传标记用于识别大群体中个体基因组成的差异。在作物植物中，遗传标记对于培育改良作物非常重要，因为它们可以用来追踪亲本对后代的贡献。这使育种者能够快速识别具有理想性状的DNA区域，并将这些区域培育成品系，同时消除不需要的区域。作物育种需要对许多植物进行数年的基因组成筛选。使用的遗传标记越多，育种就越精确，减少了育种过程中的时间和不确定性。提高植物育种的效率和可预测性对于确保小麦等主要作物的未来供应至关重要。我们的目标是利用我们在小麦基因组学和下一代测序方面的专业知识，与英国小麦育种家合作开发高通量小麦基因分型。

项目成果

Genome sequence of the progenitor of the wheat D genome Aegilops tauschii.

• DOI: 10.1038/nature24486
• 发表时间: 2017-11-23
• 期刊: Nature
• 影响因子: 64.8
• 作者: Luo MC;Gu YQ;Puiu D;Wang H;Twardziok SO;Deal KR;Huo N;Zhu T;Wang L;Wang Y;McGuire PE;Liu S;Long H;Ramasamy RK;Rodriguez JC;Van SL;Yuan L;Wang Z;Xia Z;Xiao L;Anderson OD;Ouyang S;Liang Y;Zimin AV;Pertea G;Qi P;Bennetzen JL;Dai X;Dawson MW;Müller HG;Kugler K;Rivarola-Duarte L;Spannagl M;Mayer KFX;Lu FH;Bevan MW;Leroy P;Li P;You FM;Sun Q;Liu Z;Lyons E;Wicker T;Salzberg SL;Devos KM;Dvořák J
• 通讯作者: Dvořák J

An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations.

• DOI: 10.1101/gr.217117.116
• 发表时间: 2017-05
• 期刊: Genome research
• 影响因子: 7
• 作者: Clavijo BJ;Venturini L;Schudoma C;Accinelli GG;Kaithakottil G;Wright J;Borrill P;Kettleborough G;Heavens D;Chapman H;Lipscombe J;Barker T;Lu FH;McKenzie N;Raats D;Ramirez-Gonzalez RH;Coince A;Peel N;Percival-Alwyn L;Duncan O;Trösch J;Yu G;Bolser DM;Namaati G;Kerhornou A;Spannagl M;Gundlach H;Haberer G;Davey RP;Fosker C;Palma FD;Phillips AL;Millar AH;Kersey PJ;Uauy C;Krasileva KV;Swarbreck D;Bevan MW;Clark MD
• 通讯作者: Clark MD