权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

PATHOPHYSIOLOGIC MECHANISMS IN OCULAR HYPOTONY

眼肌张力减退的病理生理机制

基本信息

批准号：
3257587
负责人：
HERBERT L CANTRILL
金额：
$ 9.44万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-07-01 至 1988-12-31
项目状态：
已结题

项目摘要

Parallel human and primate studies of the pathophysiologic mechanisms of ocular hypotony in eyes with ciliochoroidal detachment, cyclodialysis, iridocyclitis, and retinal detachment will be undertaken. Primate models of each of these disorders have been developed. Previous studies have indicated a key role for uveoscleral outflow in the pathogenesis of hypotony. Therefore, characterization of the physiology and anatomy of the uveoscleral outflow pathway will be performed. The intraocular pressure dependence, suprachoroidal hydrostatic pressure, and permeability of the ciliary musle interstitial spaces will be evaluated with fluorophotometry and flurorescence microccopy in normal eyes. The extravascular protein concentration and vascular permeability of the uvea will also be determined. In monkey eyes with ciliochoroidal detachment of iridocyclitis, the uveoscleral outflow rate and extravascular protein concentration and ciliary muscle permeability will be measured. The underlying hypothesis to be studied in the pathogenesis of hypotony is increased fluid permeability of the ciliary muscle interstitial spaces due to an increase in extravascular protein concentration due to abnormal vascular permeability. In a model of chronic rhegmatogenous retinal detachment, previous studies have strongly argued for the presence of posterior fluid movement through the virtreous, into the subretinal space, and across the RPE. From vitreous fluorophotometry, measurement of the inward and outward permeability of the blood retinal barrier (RPE) of carboxyfluorescein allows calculation of five physiologic parameters: (1) diffusional permeability of RPE to carboxyfluorescein, (2) rate of solute-linked water transport, (3) osmotic fluid permeability of the RPE, (4) hydrostatic fluid permeability of the RPE, and (5) active carboxyfluorescein transport of the RPE. These parameters will be measured in monkeys with normal eyes, eyes with acute, sub-acute, or chronic retinal detachment, following cryopexy, and after retinal detachment repair. The effects of various drugs will also be studied. Patients with hypotony from ciliochoroidal detachment, cyclodialysis or chronic iridocyclitis will be studied with aqueous fluorophotometry to measure the aqueos flow rate. The episcleral venous pressure will also be measured. In summary, the pathophysiologic mechanisms of ocular hypotony will be studied with special emphasis on unconventional aqueous outflow pathways.

人类和灵长类动物的病理生理学研究睫状脉络膜炎眼低眼压的发生机制视网膜脱离、睫状体脱离、虹膜睫状体炎和视网膜脱离将进行。这些疾病的灵长类模型已经被开发出来。以前的研究表明，在低眼压的发病机制中葡萄膜巩膜外流。因此，表征的生理和解剖的将进行葡萄膜巩膜流出路径。眼内压力依赖性，脉络膜上静水压力，和睫状肌间隙的渗透性将是用荧光光度法和荧光显微镜评价正常的眼睛血管外蛋白浓度和还将确定葡萄膜的血管渗透性。在猴眼虹膜睫状体炎睫状体脉络膜脱离，葡萄膜巩膜流出率和血管外蛋白浓度并测量睫状肌渗透性。底层在张力减退的发病机制中要研究的假设是睫状肌间质的液体渗透性增加由于血管外蛋白浓度增加而导致的间隙因为异常的血管渗透性。在慢性孔源性视网膜脱离模型中，以前的研究强烈认为，后流体运动通过virtreous，进入视网膜下间隙和穿过视网膜色素上皮。从玻璃体荧光光度法，测量向内和向外血视网膜屏障（RPE）的通透性羧基荧光素可以计算五种生理参数：（1）RPE对羧基荧光素，（2）溶质连接的水转运速率，（3） RPE的渗透液渗透性，（4）流体静力学 RPE的通透性，和（5）活性羧基荧光素 RPE的运输。这些参数将在正常眼猴，急性、亚急性或慢性眼视网膜脱离，冷冻固定术后，视网膜脱离后，脱离修复各种药物的作用也将研究了睫状体脉络膜脱离引起张力减退的患者，睫状体分离或慢性虹膜睫状体炎将用水性角膜基质进行研究。荧光光度法测量水的流速。巩膜还将测量静脉压。总而言之，将研究眼低眼压的病理生理机制特别强调非常规水流出途径。