Re-engineering robustness in to industrial antibiotic producing Streptomyces strains

重新设计工业抗生素生产链霉菌菌株的稳健性

• 批准号: BB/T004126/1
• 负责人: Paul Hoskisson
• 金额: $ 45.79万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT004126%2F1
• 关键词: Re; engineering; robustness; industrial; antibiotic

Currently around two-thirds of our antibiotics are made by the soil bacterium Streptomyces. Industrial Streptomyces usually undergo several rounds of random mutagenesis to improve their production characteristics becoming highly-adapted to industrial culture conditions. There is a problem, however, highly-adapted strains are less flexible limiting alterations to production processes and the introduction of sustainable feedstocks. This project will identify genes used by the bacteria to adapt to certain feedstocks and develop new, rapid methods to repair genes lost or damaged genes during mutagenesis, maintaining performance and increasing the flexibility of high-producing strains. This is important because the generation of industrial strains is time consuming and labour intensive. Understanding what limits flexibility in high-yielding antibiotic producers and repairing these mutations will make it easier and greener to make future antibiotics and combat antimicrobial resistance.

目前，我们大约三分之二的抗生素是由土壤细菌制造的。工业链霉菌通常会经历几轮随机诱变，以提高其生产特征，使其高度适应工业文化条件。但是，有一个问题，高度适应的菌株对生产过程和引入可持续原料的限制更为灵活的限制。该项目将确定细菌用于适应某些原料的基因，并开发新的快速方法来修复诱变期间损失或受损的基因，保持性能并提高高产生菌株的灵活性。这很重要，因为工业菌株的产生是耗时和劳动量的。了解什么限制了高产物抗生素生产商的灵活性并修复这些突变将使未来的抗生素和打击抗菌耐药性变得更加绿色。

项目成果

Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness

• DOI: 10.1101/2021.12.17.473116
• 发表时间: 2022-03-31
• 期刊: bioRxiv
• 作者: Bottura, B.;Rooney, L. M.;McConnell, G.
• 通讯作者: McConnell, G.

Cryptic or Silent? The Known Unknowns, Unknown Knowns, and Unknown Unknowns of Secondary Metabolism.

• DOI: 10.1128/mbio.02642-20
• 发表时间: 2020-10-20
• 期刊: mBio
• 影响因子: 6.4
• 作者: Hoskisson PA;Seipke RF
• 通讯作者: Seipke RF

Optimisation of aurodox production by Streptomyces goldiniensis.

金链霉菌 Aurodox 生产的优化。

• DOI: 10.1099/acmi.0.000572.v1
• 发表时间: 2023
• 作者: E McHugh R

Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness.

• DOI: 10.1016/j.bioflm.2022.100084
• 发表时间: 2022-12
• 期刊: Biofilm
• 影响因子: 6.8

Editorial overview: Eco-evolutionary dynamics in microbiology comes of age.

编辑概述：微生物学的生态进化动力学已经成熟。

• DOI: 10.1016/j.mib.2022.102171
• 发表时间: 2022
• 期刊: Current opinion in microbiology
• 影响因子: 5.4
• 作者: Bruce J