Re-engineering robustness in to industrial antibiotic producing Streptomyces strains

重新设计工业抗生素生产链霉菌菌株的稳健性

• 批准号: BB/T004126/1
• 负责人: Paul Hoskisson
• 金额: $ 45.79万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT004126%2F1
• 关键词: Re; engineering; robustness; industrial; antibiotic

Currently around two-thirds of our antibiotics are made by the soil bacterium Streptomyces. Industrial Streptomyces usually undergo several rounds of random mutagenesis to improve their production characteristics becoming highly-adapted to industrial culture conditions. There is a problem, however, highly-adapted strains are less flexible limiting alterations to production processes and the introduction of sustainable feedstocks. This project will identify genes used by the bacteria to adapt to certain feedstocks and develop new, rapid methods to repair genes lost or damaged genes during mutagenesis, maintaining performance and increasing the flexibility of high-producing strains. This is important because the generation of industrial strains is time consuming and labour intensive. Understanding what limits flexibility in high-yielding antibiotic producers and repairing these mutations will make it easier and greener to make future antibiotics and combat antimicrobial resistance.

目前，大约三分之二的抗生素是由土壤细菌链霉菌产生的。工业链霉菌通常经过多轮随机诱变以提高其生产特性，使其高度适应工业培养条件。然而，存在一个问题，高度适应的菌株不太灵活，限制了对生产工艺的改变和可持续原料的引入。该项目将确定细菌用于适应某些原料的基因，并开发新的快速方法来修复突变过程中丢失或受损的基因，保持性能并增加高产菌株的灵活性。这一点很重要，因为工业菌株的产生既耗时又耗力。了解是什么限制了高产抗生素生产商的灵活性，并修复这些突变，将使未来的抗生素生产更容易，更环保，并对抗抗生素耐药性。

项目成果

Optimisation of aurodox production by Streptomyces goldiniensis.

金链霉菌 Aurodox 生产的优化。

• DOI: 10.1099/acmi.0.000572.v1
• 发表时间: 2023
• 作者: E McHugh R

Cryptic or Silent? The Known Unknowns, Unknown Knowns, and Unknown Unknowns of Secondary Metabolism.

• DOI: 10.1128/mbio.02642-20
• 发表时间: 2020-10-20
• 期刊: mBio
• 影响因子: 6.4
• 作者: Hoskisson PA;Seipke RF
• 通讯作者: Seipke RF

Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness.

• DOI: 10.1016/j.bioflm.2022.100084
• 发表时间: 2022-12
• 期刊: Biofilm
• 影响因子: 6.8

Editorial overview: Eco-evolutionary dynamics in microbiology comes of age.

编辑概述：微生物学的生态进化动力学已经成熟。

• DOI: 10.1016/j.mib.2022.102171
• 发表时间: 2022
• 期刊: Current opinion in microbiology
• 影响因子: 5.4
• 作者: Bruce J

Microbial Primer: Bacterial growth kinetics This article is part of the Microbial Primers collection.

微生物底漆：细菌生长动力学本文是微生物底漆系列的一部分。

• DOI: 10.1099/mic.0.001428
• 发表时间: 2024
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: Fernández-Martínez L