Enhancing the yield of industrial Actinomycete fermentations

提高工业放线菌发酵的产量

• 批准号: BB/N023544/1
• 负责人: Paul Hoskisson
• 金额: $ 88.78万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN023544%2F1
• 关键词: Enhancing; yield; industrial; Actinomycete; fermentations

A new and radical approach to strain construction is needed for the UK to remain competitive in manufacturing off-patent antibiotics. Antibiotic fermentation processes balance nutrients and cell growth rate with the chemical and physical environments. Cellular output is traditionally improved by selecting higher-producing mutants and optimising fermentation media. However, many antibiotics are unstable in the extracellular environment, with well characterised relationships between pH and degradation rate. The pH range for operation of the biological system often conflicts with the optimum for chemical stability of the product. For an established commercial process we propose to utilise recent advances in synthetic biology and genomics to develop a bacterial strain and industrial fermentation process capable of operating at a lower pH, facilitating stability of the desired product.

英国需要一种新的、激进的菌株构建方法，以保持在生产非专利抗生素方面的竞争力。抗生素发酵过程平衡营养和细胞生长速率与化学和物理环境。传统上通过选择高产突变体和优化发酵培养基来提高细胞产量。然而，许多抗生素在细胞外环境中是不稳定的，pH和降解速率之间具有良好的特征关系。生物系统运行的pH值范围通常与产品的最佳化学稳定性相冲突。对于已建立的商业工艺，我们建议利用合成生物学和基因组学的最新进展来开发能够在较低pH下操作的细菌菌株和工业发酵工艺，从而促进所需产品的稳定性。

项目成果

ActDES - a Curated Actinobacterial Database for Evolutionary Studies

ActDES - 用于进化研究的精选放线菌数据库

• DOI: 10.1101/2020.05.20.105536
• 发表时间: 2020
• 作者: Schniete J

ActDES - a curated Actinobacterial Database for Evolutionary Studies.

• DOI: 10.1099/mgen.0.000498
• 发表时间: 2021-01
• 期刊: Microbial genomics
• 影响因子: 3.9
• 作者: Schniete JK;Selem-Mojica N;Birke AS;Cruz-Morales P;Hunter IS;Barona-Gomez F;Hoskisson PA
• 通讯作者: Hoskisson PA

Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences.

• DOI: 10.1038/s41598-018-35005-2
• 发表时间: 2018-11-12
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Albanna A;Sim M;Hoskisson PA;Gillespie C;Rao CV;Aldridge PD
• 通讯作者: Aldridge PD

Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences

使用来自大肠杆菌的 flhDC 驱动肠沙门氏菌 sv Typhimurium flagellum 的表达，导致关键的调控和细胞差异

• DOI: 10.1101/333146
• 发表时间: 2018
• 作者: Albanna A